AB204519

Mouse CXCL11 ELISA Kit (I-TAC)

Recombinant
SimpleStep
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(3 Publications)

Mouse CXCL11 ELISA Kit (I-TAC) is a single-wash 90-min Simplestep used to quantify Mouse CXCL11 (I-TAC) with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Scyb11, Cxcl11, C-X-C motif chemokine 11, Interferon-inducible T-cell alpha chemoattractant, Small-inducible cytokine B11, I-TAC

3 Images

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

Interpolated concentrations of CXCL11 in mouse serum and plasma.

Heparin appears to release erythrocyte bound CXCL11, resulting in higher concentration than plasma samples collected with EDTA or sodium citrate.

sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

Interpolated concentrations of mouse CXCL11 in RAW264.7 cell culture supernatant (unstimulated and LPS stimulated) and mouse lung supernatant (stimulated with LPS and PMA).

The concentrations of CXCL11 were measured in duplicate and interpolated from the CXCL11 standard curve and corrected for sample dilution.

sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

Example of CXCL11 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.4 pg/mL

Range

7.8 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse CXCL11 ELISA Kit (I-TAC) (ab204519) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL11 (I-TAC) protein in cell culture supernatant, plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL11 (I-TAC) with 1.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CXCL11 (I-TAC) is a chemokine for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment and in skin immune responses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "110 - 120 %", "average": "= 115" }, { "sample": "Serum", "range": "107 - 116 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "116 - 121 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "94 - 122 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "86 - 92 %", "average": "= 90" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab204519-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB204519-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB204519-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB204519-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB204519-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB204519-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB204519-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB204519-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CXCL11 Lyophilized Recombinant Protein", "number":"AB204519-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXLC11 Capture Antibody", "number":"AB204519-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB204519-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXLC11 Detector Antibody", "number":"AB204519-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL11 also known as I-TAC or Interferon-inducible T-cell alpha chemoattractant is a small cytokine belonging to the CXC chemokine family. It weighs around 8 to 11 kilodaltons. CXCL11 predominantly expresses itself in tissues such as the thymus spleen and peripheral blood leukocytes after stimulation with interferons. Its primary role is attracting activated T-cells especially those expressing CXCR3 receptor contributing to immune surveillance and response.

Biological function summary

The chemokine plays an important role in immune system regulation and inflammation response. It attracts and activates leukocytes including T-cells and natural killer cells driving them to sites of inflammation and injury. CXCL11 is not part of a complex but interacts with other chemokines and cytokines to modulate immune responses. Its expression is upregulated in response to IFN-γ working synergistically with other chemokines like CXCL9 and CXCL10 in inflammatory environments.

Pathways

CXCL11 is integral to the chemotaxis signaling pathway. It influences the migration and activation of immune cells by interacting with the CXCR3 receptor. This pathway shares involvement with proteins such as CXCL10 which also binds to CXCR3 enabling precise directionality and regulation of immune cell movement towards inflammation sites. Additionally CXCL11's participation in the inflammatory response pathway connects it to multiple cellular responses necessary for combating infections.

CXCL11 has been linked to autoimmune diseases and cancer. Its elevated expression is associated with conditions like multiple sclerosis where it may contribute to the pathological migration of immune cells into the central nervous system. In cancer CXCL11 can affect tumor microenvironments by recruiting immune cells with cytotoxic potential although this may also facilitate tumor progression in certain contexts. Proteins related to its disease association include CXCR3 and CXCL10 which together modulate various immunological outcomes in these diseases.

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Product protocols

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Target data

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses (By similarity).

See full target information Cxcl11

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:226 PubMed35222703

2022

Inhibition of thioredoxin-interacting protein may enhance the therapeutic effect of dehydrocostus lactone in cardiomyocytes under doxorubicin stimulation via the inhibition of the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zhang,Cuiyu Chu,Yuankun Huang

Nature 557:112-117 PubMed29695863

2018

LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nieves Peltzer,Maurice Darding,Antonella Montinaro,Peter Draber,Helena Draberova,Sebastian Kupka,Eva Rieser,Amanda Fisher,Ciaran Hutchinson,Lucia Taraborrelli,Torsten Hartwig,Elodie Lafont,Tobias L Haas,Yutaka Shimizu,Charlotta Böiers,Aida Sarr,James Rickard,Silvia Alvarez-Diaz,Michael T Ashworth,Allison Beal,Tariq Enver,John Bertin,William Kaiser,Andreas Strasser,John Silke,Philippe Bouillet,Henning Walczak

Trends in cell biology 26:445-461 PubMed26877205

2016

Holding RIPK1 on the Ubiquitin Leash in TNFR1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nieves Peltzer,Maurice Darding,Henning Walczak

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CXCL11 ELISA Kit (I-TAC)

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

Sandwich ELISA - Mouse CXCL11 ELISA Kit (I-TAC) (AB204519)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise