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AB193720

Mouse CXCL15 ELISA Kit

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(1 Publication)

Mouse CXCL15 ELISA Kit is a Sandwich ELISA for the measurement of Mouse CXCL15 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Scyb15, Cxcl15, C-X-C motif chemokine 15, Lungkine, Small-inducible cytokine B15

1 Images
Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB193720)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB193720)

Typical Standard Curve using ab193720 CXCL15 Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.8 ng/mL

Range

0.8 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CXCL15 mouse ELISA Kit (ab193720) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL15 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse CXCL15 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CXCL15 present in the samples. The wells are washed and biotinylated anti-mouse CXCL15 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CXCL15 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "102 - 128 %", "average": "= 120.1" }, { "sample": "Plasma", "range": "118 - 150 %", "average": "= 134" }, { "sample": "Cell culture media", "range": "124 - 144 %", "average": "= 135.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193720-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193720-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB193720-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193720-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193720-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse CXCL15 Microplate (12 strips x 8 wells)", "number":"AB193720-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse CXCL15 Standards (lyophilized)", "number":"AB193720-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse CXCL15 detection antibody", "number":"AB193720-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193720-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB193720-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL15 also known as lungkine is a small cytokine belonging to the CXC chemokine family. This protein exhibits a molecular mass of approximately 9 kDa. CXCL15 is expressed mainly in epithelial cells of the lung where it facilitates the recruitment of neutrophils. Unlike some other cytokines lungkine is produced constitutively and does not require induction by inflammatory stimuli. Its role as a chemokine indicates a specific involvement in respiratory immune responses.
Biological function summary

CXCL15 is involved in the recruitment and activation of neutrophils playing a pivotal role in the innate immune defense. It does not form a complex; instead it functions as a soluble signal guiding the migration of immune cells to sites of inflammation in the lungs. CXCL15 contributes to the body's defense mechanisms against infection. Its interaction with neutrophils is important for maintaining pulmonary health and responding to pathogenic challenges.

Pathways

The recruitment activity of CXCL15 is involved in chemokine signaling and immune response pathways. CXCL15 interacts with G protein-coupled receptors on neutrophils to drive chemotaxis the directed movement of cells toward areas of infection. This pathway involves cooperation with other cytokines such as CXCL1 and CXCL8 which also play roles in neutrophil trafficking and activation in inflammatory scenarios.

CXCL15 has a relationship with chronic obstructive pulmonary disease (COPD) and acute lung injury. Elevated levels of CXCL15 may exacerbate inflammatory conditions contributing to the progression and severity of these diseases. In COPD CXCL15 influences neutrophil accumulation in the airways often linked with other chemokines like IL-8. Understanding its role may offer insight into therapeutic strategies targeting excessive neutrophilic infiltration in lung-related disorders.
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Product protocols

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Target data

Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions.
See full target information Cxcl15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 16:5095-5109 PubMed38026263

2023

LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi Chen
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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