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AB234567

Mouse CXCL15 ELISA Kit

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Mouse CXCL15 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL15 with a sensitivity of 1.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Scyb15, Cxcl15, C-X-C motif chemokine 15, Lungkine, Small-inducible cytokine B15

4 Images
Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)

Example of mouse CXCL15 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)

Example of mouse CXCL15 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)

Interpolated concentrations of native CXCL15 in mouse plasma -heparin, lung supernatant and bronchoalveolar lavage samples.

The concentrations of CXCL15 were measured in duplicates, interpolated from the CXCL15 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma - heparin 50%, lung supernatant 10% and bronchoalveolar lavage 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL15 concentration was determined to be 757.6 pg/mL in neat plasma – heparin, 13354.54 pg/mL in neat lung supernatant, and 5334.90 pg/mL in neat bronchoalveolar lavage.

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL15 ELISA Kit (AB234567)

Interpolated concentrations of native CXCL15 in mouse lung extract based on a 500 μg/mL extract load.

The concentrations of CXCL15 were measured in duplicate and interpolated from the CXCL15 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL15 concentration was determined to be 325.81 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.7 pg/mL

Range

25 - 4800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CXCL15 ELISA Kit (ab234567) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL15 protein in other biological fluids, tissue extracts, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL15 with 1.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Lung Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Lung Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Other biological fluids", "range": "114 - 120 %", "average": "= 118" }, { "sample": "Tissue Extracts", "range": "108 - 109 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "91 - 93 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234567-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234567-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234567-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234567-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234567-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234567-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234567-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CXCL15 Lyophilized Recombinant Protein", "number":"AB234567-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL15 Capture Antibody", "number":"AB234567-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB234567-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234567-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL15 Detector Antibody", "number":"AB234567-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234567-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL15 also known as lungkine is a small cytokine belonging to the CXC chemokine family. This protein exhibits a molecular mass of approximately 9 kDa. CXCL15 is expressed mainly in epithelial cells of the lung where it facilitates the recruitment of neutrophils. Unlike some other cytokines lungkine is produced constitutively and does not require induction by inflammatory stimuli. Its role as a chemokine indicates a specific involvement in respiratory immune responses.
Biological function summary

CXCL15 is involved in the recruitment and activation of neutrophils playing a pivotal role in the innate immune defense. It does not form a complex; instead it functions as a soluble signal guiding the migration of immune cells to sites of inflammation in the lungs. CXCL15 contributes to the body's defense mechanisms against infection. Its interaction with neutrophils is important for maintaining pulmonary health and responding to pathogenic challenges.

Pathways

The recruitment activity of CXCL15 is involved in chemokine signaling and immune response pathways. CXCL15 interacts with G protein-coupled receptors on neutrophils to drive chemotaxis the directed movement of cells toward areas of infection. This pathway involves cooperation with other cytokines such as CXCL1 and CXCL8 which also play roles in neutrophil trafficking and activation in inflammatory scenarios.

CXCL15 has a relationship with chronic obstructive pulmonary disease (COPD) and acute lung injury. Elevated levels of CXCL15 may exacerbate inflammatory conditions contributing to the progression and severity of these diseases. In COPD CXCL15 influences neutrophil accumulation in the airways often linked with other chemokines like IL-8. Understanding its role may offer insight into therapeutic strategies targeting excessive neutrophilic infiltration in lung-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions.
See full target information Cxcl15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:85-102 PubMed32623586

2020

RP11-323N12.5 promotes the malignancy and immunosuppression of human gastric cancer by increasing YAP1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Wang,Feng Huang,Yaxiang Shi,Qinghui Zhang,Song Xu,Yongliang Yao,Runqiu Jiang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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