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AB100677

Mouse CXCL16 ELISA Kit

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(2 Publications)

Mouse CXCL16 ELISA Kit is a Sandwich ELISA for the measurement of Mouse CXCL16 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Srpsox, Cxcl16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX

2 Images
Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB100677)
  • sELISA

Unknown

Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB100677)

Representative standard curve using ab100677

Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB100677)
  • sELISA

Unknown

Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB100677)

Representative standard curve using ab100677

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1.3 pg/mL

Range

1.25 - 80 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CXCL16 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL16 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse CXCL16 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 103 %", "average": "= 96.71" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 94.34" }, { "sample": "Plasma", "range": "82 - 102 %", "average": "= 93.49" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100677-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100677-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100677-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100677-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse CXCL16 Standard (lyophilized)", "number":"AB100677-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CXCL16 Microplate (12 x 8 wells)", "number":"AB100677-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100677-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100677-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse CXCL16", "number":"AB100677-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100677-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.
Biological function summary

CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.

Pathways

CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.

CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.
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Product protocols

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Target data

Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis.
See full target information Cxcl16
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging and disease 12:786-800 PubMed34094642

2021

Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Wu,Zhuoze Wu,Aodi He,Tongmei Zhang,Ping Zhang,Jing Jin,Sisi Li,Gaigai Li,Xinyan Li,Shiqi Liang,Lei Pei,Rong Liu,Qing Tian,Ximiao He,Youming Lu,Zhouping Tang,Hao Li

International journal of molecular sciences 22: PubMed33669419

2021

Immunotherapy with 4-1BBL-Expressing iPS Cell-Derived Myeloid Lines Amplifies Antigen-Specific T Cell Infiltration in Advanced Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Kuriyama,Satoshi Fukushima,Toshihiro Kimura,Hisashi Kanemaru,Azusa Miyashita,Etsuko Okada,Yosuke Kubo,Satoshi Nakahara,Aki Tokuzumi,Yuki Nishimura,Ikko Kajihara,Katsunari Makino,Jun Aoi,Shinichi Masuguchi,Hirotake Tsukamoto,Takashi Inozume,Rong Zhang,Tetsuya Nakatsura,Yasushi Uemura,Satoru Senju,Hironobu Ihn
View all publications
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websiteProtocolBooklet
en
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Product promise

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