Mouse CXCL16 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(1 Publication)
Mouse CXCL16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL16 with a sensitivity of 7.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Srpsox, Cxcl16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB197744)
CXCL16 concentrations in unstimulated and LPS-stimulated RAW 264.7 cell culture supernatants.
RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. Two dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of CXCL16 adjusted for sample dilution are graphed in pg of CXCL16 per mL of supernatant (mean +/- SD, n = 2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB197744)
Example of CXCL16 standard curve prepared in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Unknown
Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB197744)
Interpolated concentrations of native CXCL16 in mouse LPS-treated RAW264.7 supernatant samples.
RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows : RAW264.7 supernatant 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Unknown
Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB197744)
Interpolated concentrations of native CXCL16 in mouse serum and plasma (citrate) samples.
The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Mouse CXCL16 ELISA Kit (AB197744)
Example of CXCL16 standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Complementary Products
Reactivity data
Product details
Mouse CXCL16 ELISA Kit (ab197744) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL16 with 7.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL16 is a chemokine. It induces a strong chemotactic response. It induces calcium mobilization. CXCL16 binds to CXCR6/Bonzo. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to oxidized low density lipoprotein, suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 is a plasma membrane protein.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.
Pathways
CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 13:7648 PubMed36496449
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com