Mouse CXCL16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL16 with a sensitivity of 7.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Nature communications 13:76482022Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErik Klapproth,Anke Witt,Pauline Klose,Johanna Wiedemann,Nikitha Vavilthota,Stephan R Künzel,Susanne Kämmerer,Mario Günscht,David Sprott,Mathias Lesche,Fabian Rost,Andreas Dahl,Erik Rauch,Lars Kattner,Silvio Weber,Peter Mirtschink,Irakli Kopaliani,Kaomei Guan,Kristina Lorenz,Paul Saftig,Michael Wagner,Ali El-ArmouchePubMed 36496449
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis.
Alternative names
Srpsox, Cxcl16, C-X-C motif chemokine 16, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16, SR-PSOX
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Recommended products
Mouse CXCL16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL16 with a sensitivity of 7.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 7.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample RAW 264.7 | n 5 | C.V. 6.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample RAW 264.7 | n 3 | C.V. 3.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 99.9 | Range 84.8 - 112.5 % |
Sample type Citrate plasma | Average % = 90.5 | Range 84.2 - 95.8 % |
Sample type Cell culture media | Average % = 96.7 | Range 86 - 108.3 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse CXCL16 ELISA Kit (ab197744) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL16 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL16 with 7.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL16 is a chemokine. It induces a strong chemotactic response. It induces calcium mobilization. CXCL16 binds to CXCR6/Bonzo. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to oxidized low density lipoprotein, suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 is a plasma membrane protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CXCL16 also known as SR-PSOX is a chemokine that functions primarily in cellular signaling. It weighs approximately 32 kDa and exists in both membrane-bound and soluble forms. Primarily found in the spleen liver lungs and kidneys CXCL16 gets highly expressed on the surface of antigen-presenting cells such as dendritic cells and macrophages. This expression enables CXCL16 to perform its role in cell migration and adhesion by binding to its receptor CXCR6.
- Biological function summary
CXCL16 plays a critical role in the immune response by mediating leukocyte migration. It acts as a chemoattractant for T cells especially those expressing the CXCR6 receptor facilitating their movement to sites of inflammation or injury. CXCL16 is not known to be part of a larger protein complex but interacts independently to moderate immune functions. It also participates in scavenging oxidized low-density lipoproteins linking it to processes in atherogenesis.
- Pathways
CXCL16 is an important player in the inflammatory and immune pathways. It is involved in the NF-kB signaling pathway which regulates inflammatory responses and the chemokine signaling pathway. Through these pathways CXCL16 closely interacts with other chemokines and cytokines such as CCR5 and IL-8 helping coordinate immune surveillance and inflammatory cascade.
- Associated diseases and disorders
CXCL16 is heavily implicated in atherosclerosis and rheumatoid arthritis. In atherosclerosis CXCL16 contributes to the accumulation of macrophages and foam cell formation in plaques. This connection associates it with inflammatory molecules like TNF-alpha. In rheumatoid arthritis CXCL16 expression is increased in synovial tissues contributing to tissue damage and inflammation potentially linked through interactions with other inflammatory mediators such as IL-6.
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5 product images
Sandwich ELISA - Mouse CXCL16 ELISA Kit (ab197744)
Example of CXCL16 standard curve prepared in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CXCL16 ELISA Kit (ab197744)
Example of CXCL16 standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse CXCL16 ELISA Kit (ab197744)
Interpolated concentrations of native CXCL16 in mouse serum and plasma (citrate) samples.
The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse CXCL16 ELISA Kit (ab197744)
Interpolated concentrations of native CXCL16 in mouse LPS-treated RAW264.7 supernatant samples.
RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows: RAW264.7 supernatant 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse CXCL16 ELISA Kit (ab197744)
CXCL16 concentrations in unstimulated and LPS-stimulated RAW 264.7 cell culture supernatants.
RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 μg/mL lipopolysaccharides (LPS) for 48 hours. Two dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of CXCL16 adjusted for sample dilution are graphed in pg of CXCL16 per mL of supernatant (mean +/- SD, n = 2).
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