AB236713

Mouse CXCL7 / PBP ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Mouse CXCL7 / PBP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL7 / PBP with a sensitivity of 0.216 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 Images

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

Interpolated concentrations of native PPBP in mouse RAW 264.7 cell supernatant (100%).

The concentrations of PPBP were measured in duplicate and interpolated from the PPBP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PPBP concentration was determined to be 41.45 pg/ml.

sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

Example of mouse PPBP standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

Interpolated concentrations of native PPBP in mouse serum, plasma and cell culture supernatant samples.

The concentrations of PPBP were measured in duplicates, interpolated from the PPBP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 160K, plasma (citrate) 1 : 80K, plasma (heparin) 1 : 20K, plasma (EDTA) 1 : 20K and RAW 264.7 cell supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PPBP concentration was determined to be 23,028.15 ng/mL in serum, 1008.09 ng/mL in plasma (citrate) and 2447.06 ng/ml in plasma (heparin), 2828.45 ng/mL in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.216 pg/mL

Range

2.73 - 175 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse CXCL7 / PBP ELISA Kit (ab236713) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL7 / PBP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL7 / PBP with 0.216 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "83 - 118 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "90 - 108 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "106 - 109 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "83 - 91 %", "average": "= 87" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab236713-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236713-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB236713-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236713-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236713-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB236713-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB236713-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL7 / PBP Capture Antibody", "number":"AB236713-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB236713-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CXCL7 / PBP Lyophilized Recombinant Protein", "number":"AB236713-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL7 / PBP Detector Antibody", "number":"AB236713-CMP03", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CXCL7 also known as PBP PBP-172 or PPBP is a chemokine with a molecular mass of around 11 kDa. It is a platelet basic protein (PBP) belonging to the family of CXC chemokines. This protein is extensively expressed in megakaryocytes and stored in the alpha-granules of platelets. Upon platelet activation CXCL7 is released and becomes a potent attractant and activator of neutrophils. It travels through the bloodstream and impacts extracellular signaling processes.

Biological function summary

The chemokine plays a significant role in the regulation of inflammation. CXCL7 is not only an individual entity but can form complexes with glycosaminoglycans on cell surfaces enhancing its functionality. This interaction helps in modulating immune responses by recruiting neutrophils and monocytes to sites of injury or infection. The chemokine contributes to wound healing and tissue repair through its involvement in the inflammatory phase.

Pathways

CXCL7 is involved in the chemokine signaling pathway and is an important mediator in the immune system's response to infections. It is related to other chemokine family proteins like CXCL8 (IL-8) which share similar pathways in inflammatory responses. These proteins interact with specific receptors on leukocytes inducing changes in the cytoskeleton and promoting chemotaxis which is the directed movement towards chemical stimuli.

CXCL7 is associated with various inflammatory conditions and has been implicated in atherosclerosis and rheumatoid arthritis. In these conditions elevated levels of this chemokine correlate with increased recruitment and activation of neutrophils exacerbating inflammation. CXCL7 also shares interactions with other proteins such as CXCL1 affecting the progression and severity of these disorders. The understanding of CXCL7's role in such pathologies offers potential therapeutic avenues for intervention.

style

left-column

Product protocols

style

left-column

Target data

See full target information CXCL7/PBP

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse CXCL7 / PBP ELISA Kit

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

Sandwich ELISA - Mouse CXCL7 / PBP ELISA Kit (AB236713)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise