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AB203364

Mouse CXCL9 ELISA Kit

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(8 Publications)

Mouse CXCL9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CXCL9 with a sensitivity of 1.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Mig, Scyb9, Cxcl9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, Protein m119, Small-inducible cytokine B9, MIG, MuMIG

6 Images
ELISA - Mouse CXCL9 ELISA Kit (AB203364)
  • ELISA

Supplier Data

ELISA - Mouse CXCL9 ELISA Kit (AB203364)
Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)

IFN-gamma treatment of Raw 264.7 cell induces secretion of MIG.

Raw 264.7 cells were cultured in the absence or presence of 500 ng/mL mouse recombinant Interferon-gamma protein for 72 hours. The cell culture supernatant samples were collected. The concentrations of MIG were measured in the diluted supernatants in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted supernatant samples were pre-diluted to 2%. The interpolated, dilution factor-corrected values are plotted in ng of MIG per mL of neat sample (mean +/- SD, n=2).

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)

IFN-gamma treatment of Raw 264.7 cell induces expression of MIG.

Raw 264.7 cells were cultured in the absence or presence of 500 ng/mL Mouse recombinant Interferon-gamma protein for 72 hours. The concentrations of MIG were measured in diluted extracts of these cells and of Mouse liver in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted Raw 264.7 cells extract samples were at 25 μg/mL. Note that 1X Diluted Mouse liver extract samples were at 500 μg/mL. The interpolated, dilution factor-corrected values are plotted in ng of MIG per mg of total protein (mean +/- SD, n=2).

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
  • sELISA

Lab

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)

Mouse MIG (CXCL9) standard curve comparison data.

Standard curve comparison between mouse MIG (CSCL9) SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)

Interpolated concentrations of MIG in mouse serum and plasma samples.

The concentrations of MIG were measured in duplicates, interpolated from the MIG standard curve and corrected for sample dilution. Note that 1X Diluted serum and plasma (citrate and EDTA) samples were pre-diluted to 50%. Note that 1X Diluted plasma (heparin) samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of MIG per mL of neat sample (mean +/- SD, n=2).

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse CXCL9 ELISA Kit (AB203364)

Example of MIG standard curve prepared in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 1.4 pg/mL

Range

6.25 - 400 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CXCL9 ELISA Kit ab203364 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse CXCL9 in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 1.4 pg/mL.

How the assay works

Mouse CXCL9 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse CXCL9 ELISA Kit ab203364 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CXCL9 antibody [EPR17005-14] - BSA and Azide free (Capture) ab242976
- Anti-CXCL9 antibody [EPR17005-127] - BSA and Azide free (Detector) ab242976

MIG (Monokine Induced by Gamma interferon) is a small cytokine belonging to the CXC motif chemokine family, thus named CXC motif chemokine family ligand 9 (CXCL9). CXCL9 is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. CXCL9 is a T-cell chemoattractant, which is induced by IFN-?.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" } ]
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Recovery

[ { "sample": "Serum", "range": "119.4 - 144 %", "average": "= 133.7" }, { "sample": "EDTA Plasma", "range": "108.3 - 125.6 %", "average": "= 115.2" }, { "sample": "Heparin Plasma", "range": "87.8 - 88.1 %", "average": "= 87.9" }, { "sample": "Citrate plasma", "range": "95.9 - 109.2 %", "average": "= 104" }, { "sample": "Cell culture media", "range": "95.3 - 106 %", "average": "= 101.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab203364-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB203364-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB203364-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB203364-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB203364-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CXCL9 Lyophilized Recombinant Protein", "number":"AB203364-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB203364-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL9 Capture Antibody", "number":"AB203364-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CXCL9 Detector Antibody", "number":"AB203364-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB203364-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB203364-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB203364-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB203364-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB203364-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCL9 also known as MIG (monokine induced by gamma interferon) is a small cytokine protein with a molecular weight of approximately 13 kDa. CXCL9 exhibits strong chemotactic properties and is secreted by various cells including endothelial cells macrophages and fibroblasts upon stimulation by interferon-gamma. Its expression primarily occurs in inflamed tissues and is associated with immune responses. Researchers often use tools like a CXCL9 ELISA kit or CXCL9 test to measure its expression levels in biological samples aiding in understanding its role in various conditions.
Biological function summary

This cytokine plays a pivotal role in the immune system by regulating leukocyte trafficking. CXCL9 exerts its function through binding to the CXCR3 receptor attracting T cells towards sites of inflammation or infection. This interaction is significant in mediating immune surveillance and host defense. CXCL9 does not form part of a larger protein complex but its chemokine activity is critical for immune system coordination. Scientific studies often highlight its involvement through CXCL9 function and expression analysis.

Pathways

CXCL9 significantly influences the chemokine signaling pathway and the Th1-type adaptive immune response. Within these pathways it interacts closely with other chemokines like CXCL10 and CXCL11 which also bind to the CXCR3 receptor. These pathways highlight the coordinated mobilization of T cells during immune challenges and inflammation emphasizing how CXCL9 is often examined alongside these related chemokines in research settings.

CXCL9 is notably associated with autoimmune diseases such as rheumatoid arthritis and inflammatory conditions like psoriasis. Its elevated expression is often observed in affected tissues contributing to disease pathogenesis through T cell migration and activation. In these contexts CXCL9 is frequently examined alongside other pro-inflammatory cytokines such as interferon-gamma and TNF-alpha which are known to modulate its expression and activity impacting disease progression and therapeutic strategies.
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Product protocols

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Target data

May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
See full target information Cxcl9
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 33:e15078 PubMed38610097

2024

Lithium chloride promotes mesenchymal-epithelial transition in murine cutaneous wound healing via inhibiting CXCL9 and IGF2.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Xiao Luo,Mianxing Wei,Bingshuai Jing,Jue Wang,Lanling Lin,Bing Shi,Qian Zheng,Chenghao Li

GeroScience 46:3889-3909 PubMed38446314

2024

Ergothioneine promotes longevity and healthy aging in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Katsube,Takahiro Ishimoto,Yutaro Fukushima,Asuka Kagami,Tsuyoshi Shuto,Yukio Kato

Oxidative medicine and cellular longevity 2022:8619275 PubMed35979397

2022

Delicaflavone Represses Lung Cancer Growth by Activating Antitumor Immune Response through N6-Methyladenosine Transferases and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xuewen Wang,Dafen Xu,Bing Chen,Dandan Huang,Zhijun Li,Yuxia Sui,Fei Lin,Hong Yao,Huang Yuan Li,Xinhua Lin

Experimental and therapeutic medicine 23:226 PubMed35222703

2022

Inhibition of thioredoxin-interacting protein may enhance the therapeutic effect of dehydrocostus lactone in cardiomyocytes under doxorubicin stimulation via the inhibition of the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhi Zhang,Cuiyu Chu,Yuankun Huang

Clinical cancer research : an official journal of the American Association for Cancer Research 27:4325-4337 PubMed34031054

2021

Opening of the Blood-Brain Barrier Using Low-Intensity Pulsed Ultrasound Enhances Responses to Immunotherapy in Preclinical Glioma Models.

Applications

Unspecified application

Species

Unspecified reactive species

Aria Sabbagh,Kevin Beccaria,Xiaoyang Ling,Anantha Marisetty,Martina Ott,Hillary Caruso,Emily Barton,Ling-Yuan Kong,Dexing Fang,Khatri Latha,Daniel Yang Zhang,Jun Wei,John DeGroot,Michael A Curran,Ganesh Rao,Jian Hu,Carole Desseaux,Guillaume Bouchoux,Michael Canney,Alexandre Carpentier,Amy B Heimberger

Frontiers in cell and developmental biology 9:653724 PubMed33816505

2021

SPTBN1 Prevents Primary Osteoporosis by Modulating Osteoblasts Proliferation and Differentiation and Blood Vessels Formation in Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejuan Xu,Jiayi Yang,Yanshi Ye,Guoqiang Chen,Yinhua Zhang,Hangtian Wu,Yuqian Song,Meichen Feng,Xiaoting Feng,Xingying Chen,Xiao Wang,Xu Lin,Xiaochun Bai,Jie Shen

Pigment cell & melanoma research 33:579-590 PubMed31894627

2020

Protective role of mouse mast cell tryptase Mcpt6 in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mirjana Grujic,Lars Hellman,Ann-Marie Gustafson,Srinivas Akula,Fabio Rabelo Melo,Gunnar Pejler

Nature communications 10:4779 PubMed31636267

2019

A human immune dysregulation syndrome characterized by severe hyperinflammation with a homozygous nonsense Roquin-1 mutation.

Applications

Unspecified application

Species

Unspecified reactive species

S J Tavernier,V Athanasopoulos,P Verloo,G Behrens,J Staal,D J Bogaert,L Naesens,M De Bruyne,S Van Gassen,E Parthoens,J Ellyard,J Cappello,L X Morris,H Van Gorp,G Van Isterdael,Y Saeys,M Lamkanfi,P Schelstraete,J Dehoorne,V Bordon,R Van Coster,B N Lambrecht,B Menten,R Beyaert,C G Vinuesa,V Heissmeyer,M Dullaers,F Haerynck
View all publications
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