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AB253223

Mouse CYR61 ELISA Kit

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(4 Publications)

Mouse CYR61 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse CYR61 with a sensitivity of 10.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
3 Images
Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)
  • sELISA

Unknown

Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)

Interpolated concentrations of native CYR61 in mouse serum, plasma (EDTA), and cell culture supernatant samples.

The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 100%, and NIH/3T3 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 209.0 pg/mL in neat serum, 119.6 pg/mL in neat plasma (EDTA), and 137.3 pg/mL in neat NIH/3T3 cell culture supernatant.

Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)
  • sELISA

Unknown

Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)

Example of mouse CYR61 standard curve in Sample Diluent NS.

The CYR61 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)
  • sELISA

Unknown

Sandwich ELISA - Mouse CYR61 ELISA Kit (AB253223)

Example of mouse CYR61 standard curve in Sample Diluent 50BP.

The CYR61 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 10.1 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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AB230947

Anti-CYR61/CCN1 antibody [EPR20681]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse CYR61 ELISA Kit (ab253223) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CYR61 protein in cell culture media, cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CYR61 with 10.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CYR61 (Cysteine-rich Angiogenic Inducer 61), also known as CCN Family Member 1 (CCN1) or Insulin-Like Growth Factor-Binding Protein 10 (IGFBP10) is a secreted protein that promotes cell proliferation, chemotaxis, angiogenesis, and cell adhesion. CYR61 exhibits both tumorigenic and tumor suppressor properties, depending in part on the localization.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 94 %", "average": "= 92" }, { "sample": "Serum", "range": "108 - 115 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "125 - 133 %", "average": "= 127" }, { "sample": "Cell culture media", "range": "83 - 95 %", "average": "= 88" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253223-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253223-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253223-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253223-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253223-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253223-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253223-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CYR61 Capture Antibody", "number":"AB253223-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BP", "number":"AB253223-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB253223-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse CYR61 Lyophilized Recombinant Protein", "number":"AB253223-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse CYR61 Detector Antibody", "number":"AB253223-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CYR61/CCN1 protein also known simply as CYR61 CCN1 or CYR61 protein is a cellular protein with a molecular weight of approximately 40 kDa. It is a member of the CCN family of regulatory proteins which includes connective tissue growth factors. CYR61 is secreted into the extracellular matrix and has a critical role in cell adhesion and signaling. The protein is expressed in various tissues with higher levels observed in the placenta lungs and kidneys reflecting its role in organ development and repair processes.
Biological function summary

The CYR61/CCN1 protein acts as a signaling molecule that influences cell proliferation migration and adhesion. It interacts with integrins and heparan sulfate proteoglycans on the cell surface facilitating cellular communication that is essential for structural and functional tissue integrity. Additionally CYR61 is a part of multiprotein complexes impacting angiogenesis and inflammation. These activities place CYR61/CCN1 at a pivotal point in wound healing and vascularization processes.

Pathways

The CYR61/CCN1 protein participates in key signaling pathways such as the MAPK/ERK and Wnt pathways. These pathways modulate gene expression related to cell growth and differentiation. CYR61 collaborates with other proteins including VEGF and FGF to regulate cardiovascular development and morphogenesis. The interaction with these pathways highlights the protein’s integral role in cellular homeostasis and response to physiological changes.

CYR61/CCN1 is linked to cancer progression and fibrotic diseases. Elevated levels of this protein are frequently found in breast cancer emphasizing its role in tumor growth and metastasis. It associates with other proteins like TGF-beta during fibrotic responses contributing to the pathology of fibrosis in organs such as the liver and lungs. The involvement in these processes highlights its potential as a therapeutic target in oncology and fibrotic disease management.
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Product protocols

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Target data

See full target information CCN family member 1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular therapeutics 2025:6813183 PubMed40225592

2025

Cysteine-Rich Protein 61 (CCN1) Deficiency Alleviated Cardiac Remodeling in 5/6 Nephrectomized Mice by Suppressing the MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihan Zhao,Liang Gu,Yunxuan Chen,Yibei Lin,Jincheng Xing,Diyan Xu,Zhen Su,Zhouqing Huang

Molecular medicine (Cambridge, Mass.) 31:124 PubMed40169980

2025

Layer-specific molecular signatures of colon anastomotic healing and leakage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hilal Sengul,Vasiliki Bantavi,Laura Gloeck,Andrew Y F Li Yim,Patrick Leven,Patrik Efferz,Bianca Schneiker,Mariola Lysson,Wouter J De Jonge,Sven Wehner

The Journal of clinical investigation 134: PubMed38598837

2024

Neuronally differentiated macula densa cells regulate tissue remodeling and regeneration in the kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Georgina Gyarmati,Urvi Nikhil Shroff,Anne Riquier-Brison,Dorinne Desposito,Wenjun Ju,Sean D Stocker,Audrey Izuhara,Sachin Deepak,Alejandra Becerra Calderon,James L Burford,Hiroyuki Kadoya,Ju-Young Moon,Yibu Chen,Markus M Rinschen,Nariman Ahmadi,Lester Lau,Daniel Biemesderfer,Aaron W James,Liliana Minichiello,Berislav V Zlokovic,Inderbir S Gill,Matthias Kretzler,János Peti-Peterdi

International journal of cancer 153:2068-2081 PubMed37602921

2023

Loss of cancer cell-derived ADAM15 alters the tumor microenvironment in colorectal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Laia Puig-Blasco,Krzysztof B Piotrowski,Signe R Michaelsen,Nicolai S Bager,Aušrinė Areškevičiūtiė,Marie-Louise Thorseth,Xiao-Feng Sun,Ulrich Auf dem Keller,Bjarne W Kristensen,Daniel H Madsen,Sebastian P Gnosa,Marie Kveiborg
View all publications
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