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AB119590

Mouse Cystatin C ELISA Kit

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(4 Publications)

Mouse Cystatin C ELISA Kit is a Sandwich ELISA for the measurement of Mouse Cystatin C in Mouse in Biofluids, Cell/Tissue Extracts, Cell Culture Media samples.

View Alternative Names

Cystatin-C, Cystatin-3, Cst3

1 Images
Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB119590)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB119590)

Representative Standard Curve using ab119590.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

312 - 20000 pg/mL

Assay Platform

Microplate

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Secondary Antibodies

AB150077

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  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's mouse Cystatin C in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Cystatin C in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

A Cystatin C specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cystatin C is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cystatin C amount of sample captured in plate.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab119590-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB119590-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB119590-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB119590-CMP08", "productcode":"" }, { "size":"1 x 4 Unit", "name":"Plate Seal", "number":"AB119590-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB119590-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB119590-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB119590-CMP04", "productcode":"" }, { "size":"2 x 20 ng", "name":"Lyophilized recombinant Mouse Cystatin C standard", "number":"AB119590-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-mouse Cystatin C antibody Microplate (12 x 8 wells)", "number":"AB119590-CMP09", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti- mouse Cystatin C antibody", "number":"AB119590-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB119590-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information Cst3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of toxicology 2024:5539447 PubMed38410096

2024

Effect of Honey and Aqueous Garlic Extracts against Short-Term Exposure of Cigarette Tobacco Smoking in Mice: Histopathological and Biochemical Investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Ziad Shraideh,Darwish Badran,Ahmed Alzbeede

Cell genomics 3:100347 PubMed37601967

2023

Cystatin C is glucocorticoid responsive, directs recruitment of Trem2+ macrophages, and predicts failure of cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sam O Kleeman,Tuba Mansoor Thakir,Breanna Demestichas,Nicholas Mourikis,Dominik Loiero,Miriam Ferrer,Sean Bankier,Yosef J R A Riazat-Kesh,Hassal Lee,Dimitrios Chantzichristos,Claire Regan,Jonathan Preall,Sarthak Sinha,Nicole Rosin,Bryan Yipp,Luiz G N de Almeida,Jeff Biernaskie,Antoine Dufour,Pinkus Tober-Lau,Arno Ruusalepp,Johan L M Bjorkegren,Markus Ralser,Florian Kurth,Vadim Demichev,Todd Heywood,Qing Gao,Gudmundur Johannsson,Viktor H Koelzer,Brian R Walker,Hannah V Meyer,Tobias Janowitz

ACS nano 9:3641-53 PubMed25790730

2015

Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Anil V Nair,Edmund J Keliher,Amanda B Core,Dennis Brown,Ralph Weissleder

Journal of the American Society of Nephrology : JASN 25:316-28 PubMed24136918

2013

Renal cells from spermatogonial germline stem cells protect against kidney injury.

Applications

FuncS

Species

Mouse

Letizia De Chiara,Sharmila Fagoonee,Andrea Ranghino,Stefania Bruno,Giovanni Camussi,Emanuela Tolosano,Lorenzo Silengo,Fiorella Altruda
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com