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AB201280

Mouse Cystatin C ELISA Kit

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(9 Publications)

Mouse Cystatin C ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Cystatin C with a sensitivity of 5.25 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Cystatin-C, Cystatin-3, Cst3

7 Images
ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • ELISA

Supplier Data

ELISA - Mouse Cystatin C ELISA Kit (AB201280)
Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)

Representative image of Mouse Cystatin C ELISA Kit (ab201280)

Components shown from left to right :

- Sample diluent NS

- 10X Wash Buffer PT (ab206977)

- 10X Mouse Cystatin Detector Antibody

- Mouse Cystatin Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Mouse Cystatin Capture Antibody (Lyophilised)

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent CP2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)

Example of Cystatin C standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • sELISA

Lab

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)

Mouse Cystatin C standard curve comparison data.

Standard curve comparison between mouse Cystatin C SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)

Titration of mouse Cystatin C expression in liver, lung, and spleen tissue extracts.

The 1X dilution for liver was an extract load of 500 μg/mL, and an extract load of 100 μg/mL for the lung and spleen extracts.

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)

Titration of mouse serum and citrate plasma within the working range of the assay.

The initial 1X dilution was 0.1%. Interpolated data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Cystatin C ELISA Kit (AB201280)

Example of Cystatin C standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.25 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Cystatin C ELISA Kit ab201280 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Cystatin C in cell culture supernatant, citrate plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 5.25 pg/mL.

How the assay works

Mouse Cystatin C SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Cystatin C ELISA Kit ab201280 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Cystatin C antibody [EPR17639-19] - BSA and Azide free (Capture) ab242743
- Anti-Cystatin C antibody [EPR17639-132] - BSA and Azide free (Detector) ab242743

Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, Cystatin C is thought to serve an important physiological role as a local regulator of this enzyme activity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.52" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.95" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 100 %", "average": "= 97" }, { "sample": "Urine", "range": "100 - 130 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "102 - 102 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "88 - 97 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab201280-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB201280-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Cystatin C Capture Antibody (lyophilized)", "number":"AB201280-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB201280-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB201280-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Cystatin C Lyophilized Recombinant Protein", "number":"AB201280-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB201280-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB201280-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB201280-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB201280-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Cystatin C Detector Antibody", "number":"AB201280-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB201280-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB201280-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
See full target information Cst3
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:7689 PubMed40044913

2025

The landscape of renal protein S-acylation in mice with lipid-induced nephrotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Fangrui Xiu,Zhibo Gai,Peter Gehrig,Witold E Wolski,Museer A Lone,Michele Visentin

Cells 13: PubMed39768208

2025

A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin W French,Joshua D Breidenbach,Shereen G Yassine,Bella Z Khatib-Shahidi,Sara Kazmi,Caitlin M Murphy,Humza S Bashir,Evan M Benson,Bivek Timalsina,Upasana Shrestha,Dhilhani Faleel,Satkeerth Boyapalli,Prabhatchandra Dube,Apurva Lad,Irum Syed,Deepak Malhotra,Amira Gohara,David J Kennedy,Steven T Haller

GeroScience 47:1653-1668 PubMed39294474

2024

An antireductant approach ameliorates misfolded proinsulin-induced hyperglycemia and glucose intolerance in male Akita mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dwight A L Mattocks,Naidu B Ommi,Virginia L Malloy,Sailendra N Nichenametla

Cell genomics 3:100347 PubMed37601967

2023

Cystatin C is glucocorticoid responsive, directs recruitment of Trem2+ macrophages, and predicts failure of cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sam O Kleeman,Tuba Mansoor Thakir,Breanna Demestichas,Nicholas Mourikis,Dominik Loiero,Miriam Ferrer,Sean Bankier,Yosef J R A Riazat-Kesh,Hassal Lee,Dimitrios Chantzichristos,Claire Regan,Jonathan Preall,Sarthak Sinha,Nicole Rosin,Bryan Yipp,Luiz G N de Almeida,Jeff Biernaskie,Antoine Dufour,Pinkus Tober-Lau,Arno Ruusalepp,Johan L M Bjorkegren,Markus Ralser,Florian Kurth,Vadim Demichev,Todd Heywood,Qing Gao,Gudmundur Johannsson,Viktor H Koelzer,Brian R Walker,Hannah V Meyer,Tobias Janowitz

Cell & bioscience 12:203 PubMed36536448

2022

VHL-recruiting PROTAC attenuates renal fibrosis and preserves renal function via simultaneous degradation of Smad3 and stabilization of HIF-2α.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Yang,Yuyi Ruan,Dan Wang,Jinjin Fan,Ning Luo,Huiting Chen,Xiaoyan Li,Wei Chen,Xin Wang

Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 33:14-24 PubMed34889809

2021

Reduced cleavage of von willebrand factor by ADAMTS13 is associated with microangiopathic acute kidney injury following trauma.

Applications

Unspecified application

Species

Unspecified reactive species

William E Plautz,Shannon H Haldeman,Mitchell R Dyer,Jason L Sperry,Francis X Guyette,Patricia A Loughran,Jurgis Alvikas,Adnan Hassoune,Lara Hoteit,Nijmeh Alsaadi,Brian S Zuckerbraun,Marian A Rollins-Raval,Jay S Raval,Roberto I Mota,Matthew D Neal

Antimicrobial agents and chemotherapy : PubMed31591120

2019

Renal glycosuria as a novel early sign of colistin-induced kidney damage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sophia L Samodelov,Michele Visentin,Zhibo Gai,Stephanie Häusler,Gerd A Kullak-Ublick

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:2212-2227 PubMed30247986

2018

Precision toxicology shows that troxerutin alleviates ochratoxin A-induced renal lipotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yang,Wentao Xu,Kunlun Huang,Boyang Zhang,Haomiao Wang,Xueqin Zhang,Lijing Gong,Yunbo Luo,Xiaoyun He

Journal of the American Society of Nephrology : JA 29:1900-1916 PubMed29925521

2018

Caspase-3 Is a Pivotal Regulator of Microvascular Rarefaction and Renal Fibrosis after Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yang,Shanshan Lan,Mélanie Dieudé,Jean-Paul Sabo-Vatasescu,Annie Karakeussian-Rimbaud,Julie Turgeon,Shijie Qi,Lakshman Gunaratnam,Natalie Patey,Marie-Josée Hébert
View all publications
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