Mouse Cystatin C ELISA Kit ab201280 is a single-wash 90-min SimpleStep ELISA used to quantify Mouse Cystatin C with a sensitivity of 5.25 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Mouse Cystatin C sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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Publications
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- Cell genomics 3:1003472023Cystatin C is glucocorticoid responsive, directs recruitment of Trem2+ macrophages, and predicts failure of cancer immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSam O Kleeman,Tuba Mansoor Thakir,Breanna Demestichas,Nicholas Mourikis,Dominik Loiero,Miriam Ferrer,Sean Bankier,Yosef J R A Riazat-Kesh,Hassal Lee,Dimitrios Chantzichristos,Claire Regan,Jonathan Preall,Sarthak Sinha,Nicole Rosin,Bryan Yipp,Luiz G N de Almeida,Jeff Biernaskie,Antoine Dufour,Pinkus Tober-Lau,Arno Ruusalepp,Johan L M Bjorkegren,Markus Ralser,Florian Kurth,Vadim Demichev,Todd Heywood,Qing Gao,Gudmundur Johannsson,Viktor H Koelzer,Brian R Walker,Hannah V Meyer,Tobias JanowitzPubMed 37601967
- Cell & bioscience 12:2032022VHL-recruiting PROTAC attenuates renal fibrosis and preserves renal function via simultaneous degradation of Smad3 and stabilization of HIF-2α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayi Yang,Yuyi Ruan,Dan Wang,Jinjin Fan,Ning Luo,Huiting Chen,Xiaoyan Li,Wei Chen,Xin WangPubMed 36536448
- Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis 33:14-242021Reduced cleavage of von willebrand factor by ADAMTS13 is associated with microangiopathic acute kidney injury following trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam E Plautz,Shannon H Haldeman,Mitchell R Dyer,Jason L Sperry,Francis X Guyette,Patricia A Loughran,Jurgis Alvikas,Adnan Hassoune,Lara Hoteit,Nijmeh Alsaadi,Brian S Zuckerbraun,Marian A Rollins-Raval,Jay S Raval,Roberto I Mota,Matthew D NealPubMed 34889809
- Antimicrobial agents and chemotherapy :2019Renal glycosuria as a novel early sign of colistin-induced kidney damage in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophia L Samodelov,Michele Visentin,Zhibo Gai,Stephanie Häusler,Gerd A Kullak-UblickPubMed 31591120
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:2212-22272018Precision toxicology shows that troxerutin alleviates ochratoxin A-induced renal lipotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Yang,Wentao Xu,Kunlun Huang,Boyang Zhang,Haomiao Wang,Xueqin Zhang,Lijing Gong,Yunbo Luo,Xiaoyun HePubMed 30247986
- Journal of the American Society of Nephrology : JA 29:1900-19162018Caspase-3 Is a Pivotal Regulator of Microvascular Rarefaction and Renal Fibrosis after Ischemia-Reperfusion Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Yang et. al.PubMed 29925521
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
23.4 - 1500 pg/mL
- Assay time
1h 30m
- Sensitivity
= 5.25 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
Alternative names
Cystatin-C, Cystatin-3, Cst3
What's included?
Recommended products
Mouse Cystatin C ELISA Kit ab201280 is a single-wash 90-min SimpleStep ELISA used to quantify Mouse Cystatin C with a sensitivity of 5.25 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Mouse Cystatin C sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
Colorimetric
- Sample types
Urine, Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
23.4 - 1500 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 5.25 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 8 | mean - | SD - | C.V. 2.95 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 3.52 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 95 - 100 % |
Sample type Urine | Average % = 111 | Range 100 - 130 % |
Sample type Citrate plasma | Average % = 102 | Range 102 - 102 % |
Sample type Cell culture media | Average % = 93 | Range 88 - 97 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse Cystatin C ELISA Kit (ab201280) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cystatin C protein in cit plasma, serum, tissue extracts, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Cystatin C with 5.25 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases. It is expressed in many organs of the body, and is an important extracellular inhibitor of cysteine proteases. As an inhibitor of cysteine proteinases, Cystatin C is thought to serve an important physiological role as a local regulator of this enzyme activity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Mouse Cystatin C ELISA Kit (ab201280)
Example of Cystatin C standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Cystatin C ELISA Kit (ab201280)
Mouse Cystatin C standard curve comparison data.
Standard curve comparison between mouse Cystatin C SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Mouse Cystatin C ELISA Kit (ab201280)
Example of Cystatin C standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Cystatin C ELISA Kit (ab201280)
Titration of mouse serum and citrate plasma within the working range of the assay.
The initial 1X dilution was 0.1%. Interpolated data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Mouse Cystatin C ELISA Kit (ab201280)
Titration of mouse Cystatin C expression in liver, lung, and spleen tissue extracts.
The 1X dilution for liver was an extract load of 500 μg/mL, and an extract load of 100 μg/mL for the lung and spleen extracts.
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