AB243678

Mouse Cytokeratin 18 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Mouse Cytokeratin 18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Cytokeratin 18 with a sensitivity of 27 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Kerd, Krt1-18, Krt18, Cytokeratin endo B, Cytokeratin-18, Keratin-18, Keratin D, CK-18, K18

9 Images

ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

ELISA

Supplier Data

ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Example of mouse Cytokeratin 18 standard curve in Sample Diluent NS.

The Cytokeratin 18 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Interpolated concentrations of native Cytokeratin 18 in mouse salivary gland and liver tissue extract based on a 5 μg/mL and 50 μg/mL extract load respectively.

The concentrations of Cytokeratin 18 were measured in duplicate and interpolated from the Cytokeratin 18 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 12,131 pg/mL in salivary gland and 1404 pg/mL in liver tissue extracts.

sELISA

Unknown

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Example of mouse Cytokeratin 18 standard curve in 1X Cell Extraction Buffer PTR.

The Cytokeratin 18 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Representative image of Mouse Cytokeratin 18 ELISA Kit (ab243678)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse Cytokeratin 18 ELISA Kit Capture Antibody

- 10X Mouse Cytokeratin 18 Detector Antibody

- 10X Mouse Cytokeratin 18 ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse Cytokeratin 18 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Interpolated concentrations of native Cytokeratin 18 in mouse serum and plasma (heparin).

The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 25%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 268 pg/mL in serum, 452 pg/mL in plasma (heparin), 332 pg/mL in plasma (EDTA) and 165 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Example of mouse Cytokeratin 18 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Example of mouse Cytokeratin 18 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Interpolated concentration of native Cytokeratin 18 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 27 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse Cytokeratin 18 ELISA Kit ab243678 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Cytokeratin 18 in heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 27 pg/mL.

How the assay works

Mouse Cytokeratin 18 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Cytokeratin 18 ELISA Kit ab243678 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Cytokeratin 18 antibody [EPR21994-271] - BSA and Azide free (Capture) ab259518
- Anti-Cytokeratin 18 antibody [EPR21994-290] - BSA and Azide free (Detector) ab259518

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "85 - 93 %", "average": "= 89" }, { "sample": "Tissue Extracts", "range": "79 - 98 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "80 - 85 %", "average": "= 82" } ]

style

left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab243678-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB243678-CMP04" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB243678-CMP06" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243678-CMP11" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB243678-CMP07" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB243678-CMP01" }, { "size":"2 x 1 Vial", "name":"Mouse Cytokeratin 18 Lyophilized recombinant Protein", "number":"AB243678-CMP08" }, { "size":"1 x 600 µL", "name":"10X Mouse Cytokeratin 18 Capture Antibody", "number":"AB243678-CMP09" }, { "size":"1 x 600 µL", "name":"10X Mouse Cytokeratin 18 Detector Antibody", "number":"AB243678-CMP10" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB243678-CMP05" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243678-CMP12" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243678-CMP13" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB243678-CMP14" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243678-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243678-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243678-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB243678-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243678-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243678-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243678-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Cytokeratin 18 Lyophilized recombinant Protein", "number":"AB243678-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB243678-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Cytokeratin 18 Capture Antibody", "number":"AB243678-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Cytokeratin 18 Detector Antibody", "number":"AB243678-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243678-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB243678-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

style

left-column

Product protocols

style

left-column

Target data

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information Krt18

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

IUBMB life 76:534-547 PubMed38380586

2024

Exosomes derived from Baicalin-pretreated bone mesenchymal stem cells improve Th17/Treg imbalance after hepatic ischemia-reperfusion via FGF21 and the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Baoyan Zhang,Linfeng Su,Zhichao Chen,Min Wu,Jianfeng Wei,Yonghua Lin

Science immunology 8:eabp9547 PubMed36735773

2023

Lung epithelial cell-derived C3 protects against pneumonia-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjaya K Sahu,Ayşe N Ozantürk,Devesha H Kulkarni,Lina Ma,Ruteja A Barve,Linus Dannull,Angel Lu,Marick Starick,Ja'Nia McPhatter,Lorena Garnica,Maxwell Sanfillipo-Burchman,Jeremy Kunen,Xiaobo Wu,Andrew E Gelman,Steven L Brody,John P Atkinson,Hrishikesh S Kulkarni

American journal of physiology. Regulatory, integr 317:R903-R911 PubMed31553663

2019

Sex modifies the consequences of extended fructose consumption on liver health, motor function, and physiological damage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Molly M Hyer,Samya K Dyer,Alix Kloster,Anum Adrees,Thomas Taetzsch,Jonathan Feaster,Gregorio Valdez,Gretchen N Neigh

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Cytokeratin 18 ELISA Kit

ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (AB243678)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Product promise