Mouse Cytokeratin 18 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Cytokeratin 18 in Tissue Extracts, Heparin Plasma, Serum samples.
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- American journal of physiology. Regulatory, integr 317:R903-R9112019Sex modifies the consequences of extended fructose consumption on liver health, motor function, and physiological damage in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMolly M Hyer et. al.PubMed 31553663
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 27 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Alternative names
Kerd, Krt1-18, Krt18, Cytokeratin endo B, Cytokeratin-18, Keratin-18, Keratin D, CK-18, K18
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Mouse Cytokeratin 18 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Cytokeratin 18 in Tissue Extracts, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.25 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 27 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 8.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 8.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 89 | Range 85 - 93 % |
Sample type Tissue Extracts | Average % = 87 | Range 79 - 98 % |
Sample type Heparin Plasma | Average % = 82 | Range 80 - 85 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Cytokeratin 18 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Cytokeratin 18 protein in mouseserum, plasma and tissue extract.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Cytokeratin 18 is a type I cytoskeletal that plays a role in filament reorganization when phosphorylated. When partnered together with Cytokeratin 8, Cytokeratin 18 is involved in IL-6 mediated barrier protection. Mutations within Cytokeratin 18 has been linked to cryptogenic cirrhosis. Cytokeratin 18 can be cleaved by caspase's generating a caspase cleaved fragment that is a marker of apoptosis and can be used as a biomarker for liver disease. This caspase cleaved Cytokeratin 18 fragment is the provided protein standard for ab243678, which also recognizes the full length total Cytokeratin 18.
Sensitivity:
The calculated minimal detectable dose (MDD) is 27 pg/mL in Sample Diluent NS.
The calculated minimal detectable dose (MDD) is 54 pg/mL in 1XCell Extraction Buffer PTR.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.
- Biological function summary
CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.
- Pathways
Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.
- Associated diseases and disorders
Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.
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4 product images
Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (ab243678)
Example of mouse Cytokeratin 18 standard curve in Sample Diluent NS.
The Cytokeratin 18 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (ab243678)
Example of mouse Cytokeratin 18 standard curve in 1X Cell Extraction Buffer PTR.
The Cytokeratin 18 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (ab243678)
Interpolated concentrations of native Cytokeratin 18 in mouse serum and plasma (heparin).
The concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 25%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 268 pg/mL in serum, 452 pg/mL in plasma (heparin), 332 pg/mL in plasma (EDTA) and 165 pg/mL in plasma (citrate).
Sandwich ELISA - Mouse Cytokeratin 18 ELISA Kit (ab243678)
Interpolated concentrations of native Cytokeratin 18 in mouse salivary gland and liver tissue extract based on a 5 μg/mL and 50 μg/mL extract load respectively.
The concentrations of Cytokeratin 18 were measured in duplicate and interpolated from the Cytokeratin 18 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 12,131 pg/mL in salivary gland and 1404 pg/mL in liver tissue extracts.
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