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AB207618

Mouse Decorin ELISA Kit

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Mouse Decorin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Decorin with a sensitivity of 0.13 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Decorin, Bone proteoglycan II, PG-S2, PG40, Dcn

4 Images
Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)

Example of mouse Decorin standard curve in Sample Diluent NS with enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)

Interpolated concentrations of Decorin in mouse serum, and plasma samples.

The concentrations of Decorin were measured in duplicates, interpolated from the Decorin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Decorin concentration was determined to be 12.3 ng/mL in serum, 10.7 ng/mL in plasma (citrate), 11.7 ng/mL in plasma (EDTA), and 19.2 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)

Example of mouse Decorin standard curve in Sample Diluent 15BS with enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Decorin ELISA Kit (AB207618)

Interpolated concentrations of spiked Decorin in mouse serum, and plasma samples.

The concentrations of Decorin were measured in duplicates, interpolated from the Decorin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, and plasma (EDTA) 25%.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.13 ng/mL

Range

0.63 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Secondary Antibodies

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Primary Antibodies

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Anti-alpha smooth muscle Actin antibody [1A4]

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Primary Antibodies

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Anti-Decorin antibody [EPR24097-105]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Decorin ELISA Kit (ab207618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Decorin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Decorin with NULL NULL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Decorin is a secreted proteoglycan of the small leucine-rich proteoglycan (SLRP) family. Decorin binds to type I and type II collagen fibrils as well as to fibronectin, TGF-beta and epidermal growth factor receptor.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.52" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 106 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "89 - 91 %", "average": "= 90" }, { "sample": "Urine", "range": "71 - 86 %", "average": "= 79" }, { "sample": "Heparin Plasma", "range": "97 - 110 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "84 - 90 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "97 - 98 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

May affect the rate of fibrils formation.
See full target information Dcn
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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