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AB155454

Mouse Decorin ELISA Kit (DCN)

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(3 Publications)

Mouse Decorin ELISA Kit (DCN) is a Sandwich (quantitative) ELISA for the measurement of Mouse Decorin (DCN) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Decorin, Bone proteoglycan II, PG-S2, PG40, Dcn

1 Images
Sandwich ELISA - Mouse Decorin ELISA Kit (DCN) (AB155454)
  • sELISA

Unknown

Sandwich ELISA - Mouse Decorin ELISA Kit (DCN) (AB155454)

Representative standard curve using ab155454.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

4.1 - 1000 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Decorin (DCN) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse Decorin in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended as anticoagulants for use in this assay).

This assay employs an antibody specific for mouse Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get better reproducibility in only 90 minutes with Mouse Decorin ELISA Kit (ab207618) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 126 %", "average": "= 104.7" }, { "sample": "Plasma", "range": "74 - 119 %", "average": "= 93.91" }, { "sample": "Cell culture media", "range": "68 - 130 %", "average": "= 111.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155454-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155454-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155454-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB155454-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse Decorin", "number":"AB155454-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Decorin Standard (lyophilized)", "number":"AB155454-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Decorin Microplate (12 x 8 wells)", "number":"AB155454-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155454-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB155454-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

May affect the rate of fibrils formation.
See full target information Dcn
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 10:96 PubMed35787306

2022

Increased CSF-decorin predicts brain pathological changes driven by Alzheimer's Aβ amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Richeng Jiang,Una Smailovic,Hazal Haytural,Betty M Tijms,Hao Li,Robert Mihai Haret,Ganna Shevchenko,Gefei Chen,Axel Abelein,Johan Gobom,Susanne Frykman,Misaki Sekiguchi,Ryo Fujioka,Naoto Watamura,Hiroki Sasaguri,Sofie Nyström,Per Hammarström,Takaomi C Saido,Vesna Jelic,Stina Syvänen,Henrik Zetterberg,Bengt Winblad,Jonas Bergquist,Pieter Jelle Visser,Per Nilsson

Nature communications 12:7122 PubMed34880260

2021

Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Jit Chatterjee,Shilpa Sanapala,Olivia Cobb,Alice Bewley,Andrea K Goldstein,Elizabeth Cordell,Xia Ge,Joel R Garbow,Michael J Holtzman,David H Gutmann

Matrix biology : journal of the International Soci 48:14-25 PubMed26344480

2015

Decorin is an autophagy-inducible proteoglycan and is required for proper in vivo autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Gubbiotti,Thomas Neill,Helena Frey,Liliana Schaefer,Renato V Iozzo
View all publications
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websiteProtocolBooklet
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Primary Antibodies

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Product promise

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