Mouse DKK1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse DKK1 with a sensitivity of 6.66 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Dickkopf-related protein 1, Dickkopf-1, Dkk-1, mDkk-1, Dkk1
- sELISA
Supplier Data
Sandwich ELISA - Mouse DKK1 ELISA Kit (AB197746)
Titration of protein spiked cell culture media within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse DKK1 ELISA Kit (AB197746)
Titration of mouse Serum and mouse plasma within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse DKK1 ELISA Kit (AB197746)
Example of DKK1 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Mouse DKK1 ELISA Kit (AB197746)
Mouse DKK1 standard curve comparison data.
Standard curve comparison between mouse DKK1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 3-fold increase in sensitivity.
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Product details
Mouse DKK1 ELISA Kit (ab197746) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK1 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse DKK1 with 6.66 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Dickkopf-related proteins are secreted proteins with two cysteine-rich domains. They are important in early vertebrate development due to their antagonistic relationship to Wnt signaling, which is involved in embryonic development, limb development, somitogenesis, and eye formation. In adults Dickkopf-related proteins are important to bone formation and bone related diseases, as well as certain cancers and Alzheimer disease. Dickkopf-related protein 1 (DKK1) is one of two secreted inhibitors of canonical Wnt signaling. DKK1 decreases Wnt signaling by inhibiting the interaction between LRP5/6 and Wnt-frizzled complexes. Elevated DKK1 levels are associated with the presence of osteolytic bone lesions in multiple myeloma patients, and may also contribute to the osteopenia and hypercalcemia often associated with myeloma. The inhibition of DKK1 has shown the reversal of the bone deteriorating patterns associated with osteoarthritis.
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Supplementary information
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Biological function summary
The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.
Pathways
DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.
Product protocols
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Publications (3)
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International journal of molecular sciences 25: PubMed38256259
2024
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Communications biology 5:1391 PubMed36539532
2022
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Molecular cancer research : MCR 19:717-725 PubMed33443105
2021
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