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AB113347

Mouse DKK1 ELISA Kit (Dickkopf-1)

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(1 Publication)

Mouse DKK1 ELISA Kit (Dickkopf-1) is a Sandwich ELISA for the measurement of Mouse DKK1 (Dickkopf-1) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Dickkopf-related protein 1, Dickkopf-1, Dkk-1, mDkk-1, Dkk1

2 Images
Sandwich ELISA - Mouse DKK1 ELISA Kit (Dickkopf-1) (AB113347)
  • sELISA

Unknown

Sandwich ELISA - Mouse DKK1 ELISA Kit (Dickkopf-1) (AB113347)

Representative standard curve using ab113347

Sandwich ELISA - Mouse DKK1 ELISA Kit (Dickkopf-1) (AB113347)
  • sELISA

Unknown

Sandwich ELISA - Mouse DKK1 ELISA Kit (Dickkopf-1) (AB113347)

Representative standard curve using ab113347

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 25 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DKK1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in plasma and cell culture supernatants. (Mouse DKK1 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Mouse DKK1 in normal serum with ab113347, only in serum spiked with Mouse DKK1.

Get better reproducibility in only 90 minutes with Mouse DKK1 ELISA Kit (ab197746) from our SimpleStep ELISA® range.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "71 - 85 %", "average": "= 79.55" }, { "sample": "Plasma", "range": "83 - 100 %", "average": "= 91.38" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113347-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113347-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB113347-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113347-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113347-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse DKK1", "number":"AB113347-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse DKK1 Standard (lyophilized)", "number":"AB113347-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"DKK1 Microplate (12 x 8 wells)", "number":"AB113347-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113347-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB113347-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
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Product protocols

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed : 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed : 18505822).
See full target information Dkk1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 8:e10480 PubMed36105473

2022

Experimental study on the effects of simvastatin in reversing the femoral metaphyseal defects induced by sodium valproate in normal and ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Ruotian Zhang,Maoxian Ren,Hedong Liu,Min Yang
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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