Mouse Dkk3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Dkk3 with a sensitivity of 17.659 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 90.625 - 5800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 17.659 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
Alternative names
Dickkopf-related protein 3, Dickkopf-3, Dkk-3, mDkk-3, Dkk3
What's included?
Recommended products
Mouse Dkk3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Dkk3 with a sensitivity of 17.659 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 90.625 - 5800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 17.659 pg/mL
- General recovery
- 101%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 4.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 101 | Range 83 - 111 % |
Sample type EDTA Plasma | Average % = 82 | Range 80 - 84 % |
Sample type Citrate plasma | Average % = 80 | Range 73 - 86 % |
Sample type Heparin Plasma | Average % = 92 | Range 86 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Dkk3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Dkk3 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse Dkk3 with 17.659 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Mouse Dkk3 ELISA Kit (ab315306)
Example of mouse Dkk3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Dkk3 ELISA Kit (ab315306)
Interpolated concentration of native Dkk3 was measured in duplicate at different sample concentrations. Undiluted samples are 1% mouse serum, plasma – citrate, plasma – EDTA, and plasma – heparin. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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