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AB285265

Mouse Anti-DNA antibody ELISA Kit

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Mouse Anti-DNA antibody ELISA Kit is a Sandwich ELISA for the measurement of Mouse Anti-DNA antibody in in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Mouse Anti-DNA antibody ELISA Kit (AB285265)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Anti-DNA antibody ELISA Kit (AB285265)

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.281 ng/mL

Range

0.469 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB236628

Anti-USP16 antibody [EPR20914-47]

RabMAb|Recombinant

Western blot - Anti-USP16 antibody [EPR20914-47] (AB236628)

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Primary Antibodies

AB27156

Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] - BSA and Azide free (AB27156)

  • 4
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Primary Antibodies

AB273137

Anti-ds DNA antibody [rDSD/4565]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [rDSD/4565] (AB273137)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Anti-DNA antibody ELISA kit (ab285265, E4355) is a sandwich ELISA assay for the quantitative measurement of Anti-DNA antibody in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of anti-DNA antibody captured from mouse samples.

Anti-dsDNA antibodies are a group of anti-nuclear antibodies (ANA) and their target antigen is double stranded DNA. Blood tests such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence are routinely performed to detect anti-dsDNA antibodies in diagnostic laboratories. They are highly diagnostic of systemic lupus erythematosus (SLE) and are implicated in the pathogenesis of lupus nephritis.

This product is manufactured by BioVision, an Abcam company and was previously called E4355 Anti-DNA antibody (Mouse) ELISA Kit. E4355-100 is the same size as the 96 test size of ab285265.

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Precision

[ { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285265-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285265-CMP05", "productcode":"" }, { "size":"1 x 5 Unit", "name":"Plate sealers", "number":"AB285265-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285265-CMP11", "productcode":"" }, { "size":"1 x 2 Vial", "name":"Lyophilized Standard", "number":"AB285265-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285265-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285265-CMP10", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285265-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample and Standard Dilution Buffer", "number":"AB285265-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285265-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285265-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antigen dilution buffer", "number":"AB285265-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com