AB264630

Mouse DPP4 ELISA Kit (CD26)

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Mouse DPP4 ELISA Kit (CD26) is a single-wash 90-min Simplestep used to quantify Mouse DPP4 (CD26) with a sensitivity of 39 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD26, Cd26, Dpp4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, T-cell activation antigen CD26, Thymocyte-activating molecule, DPP IV, THAM

3 Images

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

sELISA

Supplier Data

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

Interpolated concentrations of native DPP4 in mouse serum and plasma samples.

The concentrations of DPP4 were measured in duplicates, interpolated from the DPP4 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2%, plasma (citrate) 2%, plasma (EDTA) 2% and plasma (heparin) 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DPP4 concentration was determined to be 192 ng/mL in neat serum, 214 ng/mL in neat plasma (citrate), 213 ng/mL in neat plasma (EDTA) and 220 ng/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

Interpolated concentrations of native DPP4 in mouse urine and lung tissue culture supernatant samples.

The concentrations of DPP4 were measured in duplicates, interpolated from the DPP4 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 20%, lung tissue culture supernatant 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DPP4 concentration was determined to be 15 ng/mL in neat urine and 118 ng/mL in neat lung tissue culture supernatant

sELISA

Supplier Data

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

Example of mouse DPP4 standard curve in Sample Diluent NS.

The DPP4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 39 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse DPP4 ELISA Kit (CD26) (ab264630) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DPP4 (CD26) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse DPP4 (CD26) with 39 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

DPP4, also known as CD26, is a cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor-mediated T-cell activation. DPP4 is a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "113 - 128 %", "average": "= 120" }, { "sample": "Serum", "range": "92 - 113 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "101 - 118 %", "average": "= 110" }, { "sample": "Urine", "range": "113 - 118 %", "average": "= 116" }, { "sample": "Heparin Plasma", "range": "92 - 102 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "105 - 115 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "99 - 102 %", "average": "= 100" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264630-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264630-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264630-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264630-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB264630-CMP02", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264630-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse DPP4 (CD26) Detector Antibody", "number":"AB264630-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264630-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264630-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse DPP4 (CD26) Lyophilized Recombinant Protein", "number":"AB264630-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse DPP4 (CD26) Capture Antibody", "number":"AB264630-CMP08", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.

Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.

style

left-column

Product protocols

style

left-column

Target data

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

See full target information Dpp4

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecules and cells 47:100159 PubMed39577744

2024

Dipeptidyl peptidase 4 as an injury-responsive protein in the mouse sciatic nerve.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsoo Oh,Yongcheol Cho

Phytotherapy research : PTR 36:1297-1309 PubMed35088915

2022

A novel DPP-4 inhibitor Gramcyclin A attenuates cognitive deficits in APP/PS1/tau triple transgenic mice via enhancing brain GLP-1-dependent glucose uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Zongyang Li,Yuan Zhang,Xiangbao Meng,Min Li,Weiwei Cao,Junshan Yang,Xudong Xu,Wenlan Liu,Weiping Li,Qian Cai,Sicen Wang,Guoxu Ma,Zhiheng Liu,Guodong Huang

Medical science monitor : international medical journal of experimental and clinical research 27:e928933 PubMed33735157

2021

Functional Dissection of CD26 and Its Pharmacological Inhibition by Sitagliptin During Skin Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Jiang,Yuan Yao,Jin Li,Yanling Wang,Jie Cheng,Yumin Zhu

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse DPP4 ELISA Kit (CD26)

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

Sandwich ELISA - Mouse DPP4 ELISA Kit (CD26) (AB264630)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Product promise