Mouse E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse E-Cadherin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Breast cancer research : BCR 25:102023Tumor necroptosis-mediated shedding of cell surface proteins promotes metastasis of breast cancer by suppressing anti-tumor immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaoshan Liu,Swati Choksi,Hyung-Joon Kwon,Delong Jiao,Chengyu Liu,Zheng-Gang LiuPubMed 36703228
- International journal of molecular sciences 23:2022The Mechanism of Lipopolysaccharide's Effect on Secretion of Endometrial Mucins in Female Mice during Pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDezhang Lu,Wenxiang Hu,Tian Tian,Mengran Wang,Mengru Zhou,Chenchen WuPubMed 36077364
- Journal of healthcare engineering 2022:45740272022MiR-483 Promotes Colorectal Cancer Cell Biological Progression by Directly Targeting NDRG2 through Regulation of the PI3K/AKT Signaling Pathway and Epithelial-to-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXifeng Sun,Kun Li,Huiling Wang,Yifang Xia,Ping Meng,Xiaogang LengPubMed 35126924
- Experimental and therapeutic medicine 21:1162020MicroRNA-451a prevents cutaneous squamous cell carcinoma progression via the 3-phosphoinositide-dependent protein kinase-1-mediated PI3K/AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJixing Fu,Jianhua Zhao,Huamin Zhang,Xiaoli Fan,Wenjun Geng,Shaohua QiaoPubMed 33335579
- Journal of ovarian research 13:1222020LINC01094/miR-577 axis regulates the progression of ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Xu et. al.PubMed 33069244
- Medical science monitor : international medical jo 26:e9231892020LINC01207 Predicts Poor Prognosis and Suppresses Cell Growth and Metastasis via Regulating GSK-3β/β-Catenin Signaling Pathway in Malignant Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDapeng Chi et. al.PubMed 32533688
- American journal of physiology. Gastrointestinal a 318:G705-G7162020Severe neonatal anemia increases intestinal permeability by disrupting epithelial adherens junctions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrishnan MohanKumar et. al.PubMed 32090604
- Oncology letters 16:1801-18082018Downregulation of long non-coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Ke et. al.PubMed 30008868
- Journal of cellular and molecular medicine 22:1650-16652018GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-β1/Smad2 signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Jie Yang et. al.PubMed 29349903
- Journal of radiation research 57:370-802016Fractionated irradiation-induced EMT-like phenotype conferred radioresistance in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongfang Zhang et. al.PubMed 27125498
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.3 - 10000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 36 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity). E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity). (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.
Alternative names
CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1
What's included?
Recommended products
Mouse E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse E-Cadherin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 156.3 - 10000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 36 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 2.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 100 - 107 % |
Sample type Citrate plasma | Average % = 103 | Range 103 - 104 % |
Sample type Cell culture media | Average % = 106 | Range 103 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse E-Cadherin ELISA Kit (ab197751) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Cadherin protein in cit plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Cadherin with 36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
E-Cadherin is a member of the Cadherin family of calcium-dependent cell adhesion proteins. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
- Biological function summary
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
- Pathways
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
- Associated diseases and disorders
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
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4 product images
Sandwich ELISA - Mouse E-Cadherin ELISA Kit (ab197751)
Example of E-Cadherin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse E-Cadherin ELISA Kit (ab197751)
Example of E-Cadherin standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse E-Cadherin ELISA Kit (ab197751)
Interpolated concentrations of E-Cadherin in mouse serum and plasma (citrate).
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 364.1 ng/mL in mouse serum and 304.2 ng/mL in mouse plasma (citrate).
Sandwich ELISA - Mouse E-Cadherin ELISA Kit (ab197751)
Interpolated concentrations of native E-Cadherin in mouse liver homogenate extract based on a 250 μg/mL extract load.
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 0.42 ng/mL in mouse liver homogenate extract.
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