AB197751

Mouse E-Cadherin ELISA Kit

Recombinant
SimpleStep
What is this?

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(11 Publications)

Mouse E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse E-Cadherin with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

4 Images

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

sELISA

Supplier Data

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Example of E-Cadherin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Interpolated concentrations of E-Cadherin in mouse serum and plasma (citrate).

The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 364.1 ng/mL in mouse serum and 304.2 ng/mL in mouse plasma (citrate).

sELISA

Unknown

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Interpolated concentrations of native E-Cadherin in mouse liver homogenate extract based on a 250 μg/mL extract load.

sELISA

Supplier Data

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Example of E-Cadherin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 36 pg/mL

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse E-Cadherin ELISA Kit (ab197751) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Cadherin protein in cit plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Cadherin with 36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

E-Cadherin is a member of the Cadherin family of calcium-dependent cell adhesion proteins. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. The extracellular portion contains 5 cadherin domains that bind calcium and form homodimeric interactions. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "100 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "103 - 104 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "103 - 107 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity).. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.

See full target information Cdh1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 25:10 PubMed36703228

2023

Tumor necroptosis-mediated shedding of cell surface proteins promotes metastasis of breast cancer by suppressing anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoshan Liu,Swati Choksi,Hyung-Joon Kwon,Delong Jiao,Chengyu Liu,Zheng-Gang Liu

International journal of molecular sciences 23: PubMed36077364

2022

The Mechanism of Lipopolysaccharide's Effect on Secretion of Endometrial Mucins in Female Mice during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhang Lu,Wenxiang Hu,Tian Tian,Mengran Wang,Mengru Zhou,Chenchen Wu

Journal of healthcare engineering 2022:4574027 PubMed35126924

2022

MiR-483 Promotes Colorectal Cancer Cell Biological Progression by Directly Targeting NDRG2 through Regulation of the PI3K/AKT Signaling Pathway and Epithelial-to-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Xifeng Sun,Kun Li,Huiling Wang,Yifang Xia,Ping Meng,Xiaogang Leng

Experimental and therapeutic medicine 21:116 PubMed33335579

2020

MicroRNA-451a prevents cutaneous squamous cell carcinoma progression via the 3-phosphoinositide-dependent protein kinase-1-mediated PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jixing Fu,Jianhua Zhao,Huamin Zhang,Xiaoli Fan,Wenjun Geng,Shaohua Qiao

Journal of ovarian research 13:122 PubMed33069244

2020

LINC01094/miR-577 axis regulates the progression of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xu,Ping Zhang,Huajun Sun,Yang Liu

Medical science monitor : international medical jo 26:e923189 PubMed32533688

2020

LINC01207 Predicts Poor Prognosis and Suppresses Cell Growth and Metastasis via Regulating GSK-3β/β-Catenin Signaling Pathway in Malignant Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Chi,Wei Zhang,Yulong Jia,Damin Cong,Kui Yu,Shaoshan Hu

American journal of physiology. Gastrointestinal a 318:G705-G716 PubMed32090604

2020

Severe neonatal anemia increases intestinal permeability by disrupting epithelial adherens junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Krishnan MohanKumar,Kopperuncholan Namachivayam,Nithya Sivakumar,Natascha G Alves,Venkataramana Sidhaye,Jayanta K Das,Yerin Chung,Jerome W Breslin,Akhil Maheshwari

Oncology letters 16:1801-1808 PubMed30008868

2018

Downregulation of long non-coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Ke,Zhanwen Sun,Zhengjun Wang

Journal of cellular and molecular medicine 22:1650-1665 PubMed29349903

2018

GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-β1/Smad2 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Jie Yang,Ge-Liang Liu,Bo Liu,Tian Liu

Journal of radiation research 57:370-80 PubMed27125498

2016

Fractionated irradiation-induced EMT-like phenotype conferred radioresistance in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfang Zhang,Honglei Luo,Zhenzhen Jiang,Jing Yue,Qiang Hou,Ruifei Xie,Shixiu Wu

View all publications

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websiteProtocolBooklet

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Product promise

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Mouse E-Cadherin ELISA Kit

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (AB197751)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise