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AB100678

Mouse E-Cadherin ELISA Kit (CDH1)

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(1 Publication)

Mouse E-Cadherin ELISA Kit (CDH1) is a Sandwich (quantitative) ELISA for the measurement of Mouse E-Cadherin (CDH1) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

2 Images
Sandwich ELISA - Mouse E-Cadherin ELISA Kit (CDH1) (AB100678)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (CDH1) (AB100678)

Represntative Standard Curve using ab100678.

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (CDH1) (AB100678)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse E-Cadherin ELISA Kit (CDH1) (AB100678)

Represntative Standard Curve using ab100678.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

4.12 - 3000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's E-Cadherin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse E-Cadherin in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse E-Cadherin coated on a 96- well plate. Standards and samples are pipetted into the wells and E-Cadherin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse E-Cadherin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of E-Cadherin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Mouse E-Cadherin ELISA Kit (ab197751) from our SimpleStep ELISA® range.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 123 %", "average": "= 114.7" }, { "sample": "Serum", "range": "69 - 88 %", "average": "= 78.78" }, { "sample": "Plasma", "range": "76 - 95 %", "average": "= 88.93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100678-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100678-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100678-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"E Cadherin Microplate (12 x 8 wells)", "number":"AB100678-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100678-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100678-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100678-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100678-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse E-Cadherin Standard (lyophilized)", "number":"AB100678-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse E-Cadherin", "number":"AB100678-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
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Product protocols

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Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity).. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.
See full target information Cdh1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Circulation research 112:35-47 PubMed23071158

2012

Functional role of matrix metalloproteinase-8 in stem/progenitor cell migration and their recruitment into atherosclerotic lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Qingzhong Xiao,Feng Zhang,Luyang Lin,Changcun Fang,Guanmei Wen,Tsung-Neng Tsai,Xiangyuan Pu,David Sims,Zhongyi Zhang,Xiaoke Yin,Binia Thomaszewski,Boris Schmidt,Manuel Mayr,Ken Suzuki,Qingbo Xu,Shu Ye
View all publications
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websiteProtocolBooklet
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