AB201279

Mouse E-Selectin ELISA Kit (CD62E)

Recombinant
SimpleStep
What is this?

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(4 Publications)

Mouse E-Selectin ELISA Kit (CD62E) is a single-wash 90-min Simplestep used to quantify Mouse E-Selectin (CD62E) with a sensitivity of 8.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD62E, Elam-1, Sele, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

2 Images

Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (AB201279)

sELISA

Supplier Data

Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (AB201279)

Example of E-Selectin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (AB201279)

Titration of mouse serum and plasma samples within the working range of the assay.

The 1X dilution for serum was 4%. The 1X dilution for EDTA plasma was 0.5%, and the 1X dilution for citrate and heparin plasma was 2%. Interpolated data values (mean +/- SD, n = 2) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.55 pg/mL

Range

23.4 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse E-Selectin ELISA Kit (CD62E) (ab201279) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Selectin (CD62E) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Selectin (CD62E) with 8.55 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

E-Selectin is a cell-surface glycoprotein that plays a role in immune-adhesion. It is expressed by cytokine-stimulated endothelial cells, and mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. E-selectin is also thought to have a role in capillary morphogenesis. Its structural features include the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.13" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.57" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 89 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "85 - 99 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "97 - 106 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "90 - 100 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "84 - 103 %", "average": "= 92" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.

Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.

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Product protocols

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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1 (PubMed : 1375914). May have a role in capillary morphogenesis.

See full target information Sele

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Annals of surgery 280:570-583 PubMed38975668

2024

Codon-Optimized and de novo-Synthesized E-Selectin/AAV2 Dose-Response Study for Vascular Regeneration Gene Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca A Voza,Barry J Byrne,Yulexi Y Ortiz,Yan Li,Nga Le,Lucy Osafo,Antoine C Ribieras,Hongwei Shao,Carlos Theodore Huerta,Yuntao Wei,Gustavo Falero-Diaz,Andres Franco-Bravo,Roberta M Lassance-Soares,Roberto I Vazquez-Padron,Zhao-Jun Liu,Omaida C Velazquez

The Kaohsiung journal of medical sciences 38:565-573 PubMed35579106

2022

microRNA-145-5p attenuates acute lung injury via targeting ETS2.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Qiao,Rong-Xia Li,Shan-Gang Hu,Yu Liu,Hong-Qiang Liu,Hong-Jun Wu

Oxidative medicine and cellular longevity 2019:2197017 PubMed31781326

2019

Lipoxin A4 Ameliorates Acute Pancreatitis-Associated Acute Lung Injury through the Antioxidative and Anti-Inflammatory Effects of the Nrf2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Ye,Chenlei Zheng,Dinglai Yu,Fan Zhang,Reguang Pan,Xiaofeng Ni,Zhehao Shi,Zhongjing Zhang,Yukai Xiang,Hongwei Sun,Keqing Shi,Bicheng Chen,Qiyu Zhang,Mengtao Zhou

Scientific reports 7:12064 PubMed28935932

2017

Cannabidiol attenuates alcohol-induced liver steatosis, metabolic dysregulation, inflammation and neutrophil-mediated injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping Wang,Partha Mukhopadhyay,Zongxian Cao,Hua Wang,Dechun Feng,György Haskó,Raphael Mechoulam,Bin Gao,Pal Pacher

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse E-Selectin ELISA Kit (CD62E)

Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (AB201279)

Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (AB201279)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise