AB252368

Mouse ECM1 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Mouse ECM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse ECM1 with a sensitivity of 19 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Extracellular matrix protein 1, Secretory component p85, Ecm1

4 Images

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

sELISA

Unknown

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Interpolated concentrations of native ECM1 in 100 μg/mL mouse lung extract.

The concentrations of ECM1 were measured in duplicate and interpolated from the ECM1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 1.17 ng/mL in mouse lung extract.

sELISA

Unknown

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Example of mouse ECM1 standard curve in 1X Cell Extraction Buffer PTR.

The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Example of mouse ECM1 standard curve in Sample Diluent NS.

The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Interpolated concentrations of native ECM1 in mouse serum, plasma, and supernatant samples.

The concentrations of ECM1 were measured in duplicates, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse serum 1 : 4, mouse plasma (heparin) 1 : 5, mouse plasma (citrate) 1 : 5.7, mouse plasma (EDTA) 1 : 5, and 20% mouse lung supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 11.60 ng/mL in mouse serum, 16.53 ng/mL in mouse plasma (heparin), 19.12 ng/mL in mouse plasma (citrate) , 24.39 ng/mL in mouse plasma (EDTA), and 11.32 ng/mL in mouse lung supernatant.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 19 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse ECM1 ELISA Kit (ab252368) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ECM1 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ECM1 with 19 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ECM1 is a glycoprotein involved in the modulation of bone mineralization and plays a role in wound healing, scarring, and dermal integrity. Mutations of the ECM1 gene is the basis for lipoid proteinosis, a rare dermatologic disorder. ECM1 is currently being investigated for its role in breast cancer metastasis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "99 - 110 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "99 - 114 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "92 - 107 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "88 - 110 %", "average": "= 100" }, { "sample": "Tissue Culture Media", "range": "108 - 114 %", "average": "= 111" }, { "sample": "Tissue Extracts", "range": "102 - 110 %", "average": "= 106" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252368-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252368-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252368-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252368-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252368-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252368-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB252368-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse ECM1 Lyophilized Recombinant Protein", "number":"AB252368-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse ECM1 Capture Antibody", "number":"AB252368-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB252368-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse ECM1 Detector Antibody", "number":"AB252368-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252368-CMP06", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

style

left-column

Product protocols

style

left-column

Target data

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).

See full target information Ecm1

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse ECM1 ELISA Kit

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Sandwich ELISA - Mouse ECM1 ELISA Kit (AB252368)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Assay Kits

Assay Kits

Product promise