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AB100679

Mouse EGF ELISA Kit

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(5 Publications)

Mouse EGF ELISA Kit is a Sandwich ELISA for the measurement of Mouse EGF in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Pro-epidermal growth factor, EGF, Egf

2 Images
Sandwich ELISA - Mouse EGF ELISA Kit (AB100679)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB100679)

Representative Standard Curve using ab100679

Sandwich ELISA - Mouse EGF ELISA Kit (AB100679)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse EGF ELISA Kit (AB100679)

Representative Standard Curve using ab100679

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

3.28 - 800 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's EGF (Epidermal growth factor) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse EGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse EGF coated on a 96- well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 107 %", "average": "= 112.4" }, { "sample": "Serum", "range": "75 - 101 %", "average": "= 88.33" }, { "sample": "Plasma", "range": "80 - 101 %", "average": "= 90.23" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100679-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100679-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100679-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100679-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100679-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse EGF Standard (lyophilized)", "number":"AB100679-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100679-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse EGF", "number":"AB100679-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"EGF Microplate (12 x 8 wells)", "number":"AB100679-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100679-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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Product protocols

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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
See full target information Egf
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials 267:120412 PubMed33161320

2020

Breast cancer cells and macrophages in a paracrine-juxtacrine loop.

Applications

Unspecified application

Species

Unspecified reactive species

Sevgi Onal,Merve Turker-Burhan,Gizem Bati-Ayaz,Hamdullah Yanik,Devrim Pesen-Okvur

Journal of cellular physiology 235:3579-3591 PubMed31552684

2019

HOXC10 promotes cell migration, invasion, and tumor growth in gastric carcinoma cells through upregulating proinflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhang Li,Gangling Tong,Cheng Huang,Yunsheng Luo,Shubin Wang,Ying Zhang,Boran Cheng,Zhihong Zhang,Xuan Wu,Qiumei Liu,Min Li,Laiqing Li,Bingqiang Ni

International journal of molecular sciences 20: PubMed31557796

2019

Mesenchymal Stem Cells Extract (MSCsE)-Based Therapy Alleviates Xerostomia and Keratoconjunctivitis Sicca in Sjogren's Syndrome-Like Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ghada Abughanam,Osama A Elkashty,Younan Liu,Mohammed O Bakkar,Simon D Tran

PloS one 11:e0156198 PubMed27219328

2016

Palmitoylethanolamide Modulates Inflammation-Associated Vascular Endothelial Growth Factor (VEGF) Signaling via the Akt/mTOR Pathway in a Selective Peroxisome Proliferator-Activated Receptor Alpha (PPAR-α)-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Sarnelli,Alessandra D'Alessandro,Teresa Iuvone,Elena Capoccia,Stefano Gigli,Marcella Pesce,Luisa Seguella,Nicola Nobile,Giovanni Aprea,Francesco Maione,Giovanni Domenico de Palma,Rosario Cuomo,Luca Steardo,Giuseppe Esposito

Proceedings of the National Academy of Sciences of 109:11330-5 PubMed22733781

2012

Amniotic fluid inhibits Toll-like receptor 4 signaling in the fetal and neonatal intestinal epithelium.

Applications

Unspecified application

Species

Mouse

Misty Good,Richard H Siggers,Chhinder P Sodhi,Amin Afrazi,Feras Alkhudari,Charlotte E Egan,Matthew D Neal,Ibrahim Yazji,Hongpeng Jia,Joyce Lin,Maria F Branca,Congrong Ma,Thomas Prindle,Zachary Grant,Sapana Shah,Dennis Slagle,Jose Paredes,John Ozolek,George K Gittes,David J Hackam
View all publications
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websiteProtocolBooklet
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Product promise

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