Mouse EGFR ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse EGFR in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 19:513-5272022Ceruletide and Alpha-1 Antitrypsin as a Novel Combination Therapy for Ischemic Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Simats,Laura Ramiro,Raquel Valls,Helena de Ramón,Paula García-Rodríguez,Cyrille Orset,Laura Artigas,Teresa Sardon,Anna Rosell,Joan MontanerPubMed 35226340
- Endocrinology 158:4152-41642017Serum Quantitative Proteomic Analysis Reveals Soluble EGFR To Be a Marker of Insulin Resistance in Male Mice and Humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMayu Kyohara,Jun Shirakawa,Tomoko Okuyama,Ayuko Kimura,Yu Togashi,Kazuki Tajima,Hisashi Hirano,Yasuo TerauchiPubMed 29028997
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
1.875 - 120 ng/mL
- Assay time
1h 30m
- Sensitivity
= 60 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (PubMed:35131940).
Alternative names
Epidermal growth factor receptor, Egfr
What's included?
Recommended products
Mouse EGFR ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse EGFR in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
1.875 - 120 ng/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 60 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 1.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 97.1 - 106.7 % |
Sample type EDTA Plasma | Average % = 101.1 | Range 97.5 - 106 % |
Sample type Heparin Plasma | Average % = 96.9 | Range 95.2 - 99.7 % |
Sample type Citrate plasma | Average % = 100.5 | Range 96.2 - 105 % |
Sample type Cell culture media | Average % = 116.1 | Range 114.3 - 119.3 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Mouse EGFR ELISA Kit (ab201275) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EGFR protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse EGFR with 60 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
EGFR is a receptor tyrosine kinase that binds ligands of the EGF family and activate several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. The ligand binding triggers receptor homo- and/or hetero-dimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. EGFR activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. EGFR may also activate the NF-kappa-B signaling cascade. EGFR also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. EGFR also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Endocytosis and inhibition of the activated EGFR by phosphatases like PTPRJ and PTPRK constitute immediate regulatory mechanisms. Upon EGF-binding EGFR phosphorylates EPS15 that regulates EGFR endocytosis and activity. Moreover, inducible feedback inhibitors including LRIG1, SOCS4, SOCS5 and ERRFI1 constitute alternative regulatory mechanisms for the EGFR signaling.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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5 product images
Sandwich ELISA - Mouse EGFR ELISA Kit (ab201275)
Mouse EGFR standard curve comparison data.
Standard curve comparison between mouse EGFR SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Mouse EGFR ELISA Kit (ab201275)
Example of EGFR standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse EGFR ELISA Kit (ab201275)
Example of EGFR standard curve prepared in Sample Diluent 25BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse EGFR ELISA Kit (ab201275)
Interpolated concentrations of EGFR in mouse serum and plasma.
The concentrations of EGFR were measured in duplicates, interpolated from the EGFR standard curve and corrected for sample dilution. Note that 1X Diluted serum and plasma sample were 200X pre-diluted samples, The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse EGFR ELISA Kit (ab201275)
Interpolated concentrations of EGFR in liver cell culture supernatant samples.
The concentrations of EGFR were measured in duplicates, interpolated from the EGFR standard curve and corrected for sample dilution. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2).
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