AB205575

Mouse EMMPRIN ELISA Kit (CD147)

Recombinant
SimpleStep
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Mouse EMMPRIN ELISA Kit (CD147) is a single-wash 90-min Simplestep used to quantify Mouse EMMPRIN (CD147) with a sensitivity of 1.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD147, Basigin, Basic immunoglobulin superfamily, HT7 antigen, Membrane glycoprotein gp42, Bsg

5 Images

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

sELISA

Supplier Data

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Example of mouse EMMPRIN standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Example of mouse EMMPRIN standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Interpolated concentrations of EMMPRIN in mouse extract samples.

The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Note that 1X Diluted Raw 264.7 cell extract samples were at 5 μg/mL. Note that 1X Diluted Small Intestine extract samples were at 500 μg/mL. The interpolated, dilution factor-corrected values are plotted in pg of EMMPRIN per μg of total protein (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Example of mouse EMMPRIN standard curve in 1X Cell extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Interpolated concentrations of EMMPRIN in mouse serum, plasma, cell culture supernatant and urine samples.

EMMPRIN in mouse serum, plasma, cell culture supernatant and urine samples. The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Note that 1X Diluted serum and plasma samples were pre-diluted to 10%. Note that 1X Diluted Raw 264.7 day 3 cell culture supernatant samples were neat. Note that 1X Diluted Mouse Urine (Female) samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of EMMPRIN per mL of neat sample (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Cell culture extracts, Tissue Extracts, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.7 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse EMMPRIN ELISA Kit (CD147) (ab205575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EMMPRIN (CD147) protein in plasma, serum, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse EMMPRIN (CD147) with 1.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

EMMPRIN plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. EMMPRIN plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. EMMPRIN stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). EMMPRIN seems to be a receptor for oligomannosidic glycans. In vitro, EMMPRIN promotes outgrowth of astrocytic processes.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]

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Recovery

[ { "sample": "Serum", "range": "86.1 - 93.6 %", "average": "= 89.9" }, { "sample": "Urine", "range": "95.8 - 128.1 %", "average": "= 112.4" }, { "sample": "Cell culture media", "range": "92.4 - 98.1 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "80.6 - 87.4 %", "average": "= 83.7" }, { "sample": "EDTA Plasma", "range": "81.1 - 88.4 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "103.3 - 121.3 %", "average": "= 112.3" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.

Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.

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Product protocols

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Target data

Isoform 1. Essential for normal retinal maturation and development (PubMed : 10967074, PubMed : 11273674, PubMed : 11853760). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed : 25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed : 25957687).. Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (By similarity). Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane (PubMed : 12601063). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (By similarity). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA and KDR/VEGFR2 in endothelial cells (By similarity). Plays an important role in spermatogenesis; mediates interactions between germ cells and Sertoli cell and is essential for the development/differentiation of germ cells to round spermatids (PubMed : 11882021, PubMed : 23727514).

See full target information Bsg

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse EMMPRIN ELISA Kit (CD147)

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (AB205575)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Assay Kits

Assay Kits

Product promise