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AB193722

Mouse Endocan ELISA Kit

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(1 Publication)

Mouse Endocan ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Endocan in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Endothelial cell-specific molecule 1, ESM-1, Esm1

1 Images
Sandwich ELISA - Mouse Endocan ELISA Kit (AB193722)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endocan ELISA Kit (AB193722)

Typical Standard Curve using ab193722 Endocan Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.1 ng/mL

Range

0.1 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Endocan Mouse ELISA Kit (ab193722) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Endocan in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse Endocan coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Endocan present in the samples. The wells are washed and biotinylated anti-mouse Endocan antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Endocan bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "108 - 149 %", "average": "= 123.8" }, { "sample": "Plasma", "range": "108 - 146 %", "average": "= 121.1" }, { "sample": "Cell culture media", "range": "85 - 122 %", "average": "= 103.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193722-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193722-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193722-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse Endocan Microplate (12 strips x 8 wells)", "number":"AB193722-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193722-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse Endocan Standards (lyophilized)", "number":"AB193722-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB193722-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193722-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB193722-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse Endocan detection antibody", "number":"AB193722-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelial cell-specific molecule 1 (ESM1) also known as endocan is a proteoglycan with a mass of approximately 50 kDa. It is primarily expressed in endothelial cells of blood vessels particularly in the lungs and the kidneys. ESM1 consists of a core protein covalently attached to dermatan sulfate chains. Its expression often increases under inflammatory conditions which highlights its role in vascular biology.
Biological function summary

ESM1 acts in the regulation of cell adhesion and migration processes. It interacts with leukocytes and participates in modulating inflammatory responses. ESM1 does not form part of a multi-protein complex but its activity can influence the behavior of other cell surface molecules and receptors. This function positions ESM1 as a critical player in the dynamics of vascular endothelium.

Pathways

ESM1 modulates inflammation and angiogenesis by participating in pathways involving vascular endothelial growth factor (VEGF). It interacts with proteins such as VEGF receptor-2 impacting endothelial permeability and new blood vessel formation. These interactions situate ESM1 within the signaling networks that regulate endothelial function and vascular homeostasis.

Research associates ESM1 with conditions such as sepsis and cancer. High levels of this molecule correlate with poor prognosis in these diseases linking it functionally to VEGF and other factors involved in abnormal endothelial cell activity. Understanding ESM1's role provides insights into mechanisms of disease progression and potential therapeutic targets.
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Product protocols

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Target data

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity).
See full target information Endothelial cell-specific molecule 1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and function 38:817-825 PubMed32515027

2020

Changes of Endocan and its effect on hepatic stem cells during the rapid proliferation process of residual liver after ALPPS procedure.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Chaoliu Dai,Qi Gong
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