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AB236716

Mouse Endocan ELISA Kit

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Mouse Endocan ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Endocan with a sensitivity of 9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Endothelial cell-specific molecule 1, ESM-1, Esm1

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Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)
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Supplier Data

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)

Interpolated concentrations of spike Endocan in mouse cell culture media sample.

The concentrations of Endocan were measured in duplicates, interpolated from the Endocan standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)
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Supplier Data

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)

Interpolated concentrations of native Endocan in mouse serum and plasma samples.

The concentrations of Endocan were measured in duplicates, interpolated from the Endocan standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (citrate) 20%, plasma (EDTA) 20%, and plasma (heparin) 20%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endocan concentration was determined to be 6095 pg/mL in serum, 6649 pg/mL in plasma (citrate), 5733 pg/mL in plasma (EDTA) and 5509 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)

Example of mouse Endocan standard curve in Sample Diluent NS.

The Endocan standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endocan ELISA Kit (AB236716)

Competitor Curve Comparison.

Standard curve comparison between mouse Endocan SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 9 pg/mL

Range

31.3 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Endocan ELISA Kit (ab236716) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endocan protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Endocan with 9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]
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Recovery

[ { "sample": "Serum", "range": "117 - 117 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "111 - 116 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "103 - 115 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "108 - 110 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "82 - 85 %", "average": "= 84" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelial cell-specific molecule 1 (ESM1) also known as endocan is a proteoglycan with a mass of approximately 50 kDa. It is primarily expressed in endothelial cells of blood vessels particularly in the lungs and the kidneys. ESM1 consists of a core protein covalently attached to dermatan sulfate chains. Its expression often increases under inflammatory conditions which highlights its role in vascular biology.
Biological function summary

ESM1 acts in the regulation of cell adhesion and migration processes. It interacts with leukocytes and participates in modulating inflammatory responses. ESM1 does not form part of a multi-protein complex but its activity can influence the behavior of other cell surface molecules and receptors. This function positions ESM1 as a critical player in the dynamics of vascular endothelium.

Pathways

ESM1 modulates inflammation and angiogenesis by participating in pathways involving vascular endothelial growth factor (VEGF). It interacts with proteins such as VEGF receptor-2 impacting endothelial permeability and new blood vessel formation. These interactions situate ESM1 within the signaling networks that regulate endothelial function and vascular homeostasis.

Research associates ESM1 with conditions such as sepsis and cancer. High levels of this molecule correlate with poor prognosis in these diseases linking it functionally to VEGF and other factors involved in abnormal endothelial cell activity. Understanding ESM1's role provides insights into mechanisms of disease progression and potential therapeutic targets.
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Product protocols

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Target data

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity).
See full target information Endothelial cell-specific molecule 1
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websiteProtocolBooklet
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