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AB263894

Mouse Endostatin ELISA Kit

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Mouse Endostatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Endostatin with a sensitivity of 2.33 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Collagen alpha-1(XVIII) chain, Col18a1

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Sandwich ELISA - Mouse Endostatin ELISA Kit (AB263894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endostatin ELISA Kit (AB263894)

Interpolated concentrations of native Endostatin in mouse serum and plasma samples.

The concentrations of Endostatin were measured in duplicates, interpolated from the Endostatin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 200, plasma (EDTA) 1 : 200 and plasma (heparin) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endostatin concentration was determined to be 97.87 ng/mL in serum, 72.00 ng/mL in plasma (citrate), 85.87 ng/mL in plasma (EDTA), and 101.37 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse Endostatin ELISA Kit (AB263894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endostatin ELISA Kit (AB263894)

Interpolated concentrations of native Endostatin in mouse cell and tissue culture supernatant samples.

The concentrations of Endostatin were measured in duplicates, interpolated from the Endostatin standard curves and corrected for sample dilution. Undiluted samples are as follows : heart supernatant 1 : 8 and kidney supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endostatin concentration was determined to be 7.60 ng/mL in heart supernatant and 67.42 ng/mL in kidney supernatant.

Sandwich ELISA - Mouse Endostatin ELISA Kit (AB263894)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endostatin ELISA Kit (AB263894)

Example of mouse Endostatin standard curve in Sample Diluent NS.

The Endostatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.33 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Endostatin ELISA Kit (ab263894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endostatin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Endostatin with 2.33 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Endostatin is a cleaved product of the C-terminal domain of collagen XVIII, an endogenous angiogenesis inhibitor. Endostatin localized to wall of vasculature in the elastic fiber and basement membrane where it inhibits endothelial cell migration, in vivo and in vitro. Endostatin induces endothelial cell apoptosis and inhibits tumor growth and impairs blood vessel maturation in wound healing. It is implicated in endothelial cell adhesion and cytoskeletal organization. Mouse endostatin shares a 70, 67, 67% sequence homology with human, rat, and cow, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 115 %", "average": "= 106" }, { "sample": "Serum", "range": "94 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "94 - 102 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "86 - 96 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "101 - 105 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as Collagen XVIII alpha 1 (COL18A1) is a powerful anti-angiogenic protein with a mass around 20 kDa. It specifically inhibits the process of blood vessel formation in both in vitro and in vivo environments. This protein derives as a fragment from the carboxyl end of collagen XVIII which is a component of basement membranes. Expression of endostatin occurs primarily in the endothelial cells lining blood vessels and is also seen in tissues undergoing remodeling or repair.
Biological function summary

Endostatin functions as an important inhibitor of angiogenesis the process in which new blood vessels form from pre-existing ones. It acts independently and is not part of a larger protein complex. In the cellular microenvironment endostatin binds to various integrins inhibiting endothelial cell proliferation and inducing apoptosis. It also disrupts signal transduction pathways critical for endothelial cell survival and migration.

Pathways

Endostatin holds an important position in the angiogenesis and Wnt signaling pathways. It interacts closely with proteins like VEGF (Vascular Endothelial Growth Factor) and FGF2 (Fibroblast Growth Factor 2) where it counteracts their pro-angiogenic effects. Endostatin's role in these pathways indicates its significant impact on controlling angiogenic balance in various physiological and pathological conditions.

Endostatin exhibits notable relevance to cancer and diabetic retinopathy. In cancer the protein suppresses tumor-induced angiogenesis restricting tumor growth and metastasis. Likewise in diabetic retinopathy endostatin's inhibition of abnormal blood vessel formation in the retina offers potential therapeutic benefits. In these contexts it interacts with other key proteins like MMPs (matrix metalloproteinases) which regulate collagen degradation and release of endostatin fragments from parent collagen XVIII.
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Product protocols

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (PubMed : 9008168). Inhibits VEGFA isoform VEGF165-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2 (PubMed : 12029087). Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (PubMed : 11158588). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (By similarity).
See full target information Col18a1
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