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AB326393

Mouse Endostatin ELISA Kit, Chemiluminescent

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Mouse Endostatin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse Endostatin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.65 - 3600 pg/ml with a sensitivity of 0.69pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Collagen alpha-1(XVIII) chain, Col18a1

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Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)
  • sELISA

Lab

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)
  • sELISA

Lab

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)

Standard curve comparison between the Chemiluminescent Mouse Endostatin SimpleStep ELISA and colorimetric Mouse Endostatin SimpleStep ELISA (ab263894) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)
  • sELISA

Lab

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)

Dynamic range comparison between the Chemiluminescent Mouse Endostatin SimpleStep ELISA, colorimetric Mouse Endostatin SimpleStep ELISA (ab263894), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and broader dynamic range.

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)

Interpolated concentrations of native Endostatin in mouse serum and plasma samples.

The concentrations of Endostatin were measured in duplicates interpolated from the Endostatin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200 plasma (citrate) 1 : 200 plasma (EDTA) 1 : 200 and plasma (heparin) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Endostatin concentration was determined to be 97.87 ng/mL in serum 72.00 ng/mL in plasma (citrate) 85.87 ng/mL in plasma (EDTA) and 101.37 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Endostatin ELISA Kit, Chemiluminescent (AB326393)

Interpolated concentrations of native Endostatin in mouse cell and tissue culture supernatant samples.

The concentrations of Endostatin were measured in duplicates interpolated from the Endostatin standard curves and corrected for sample dilution. Undiluted samples are as follows : heart supernatant 1 : 8 and kidney supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Endostatin concentration was determined to be 7.60 ng/mL in heart supernatant and 67.42 ng/mL in kidney supernatant.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.69 pg/mL

Range

1.65 - 3600 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Endostatin ELISA Kit, Chemiluminescent (ab326393) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Endostatin in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.69 pg/mL.

How the assay works

Mouse Endostatin ELISA Kit, Chemiluminescent (ab326393) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Endostatin (ab326393) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Endostatin antibody [EPR22479-57] - BSA and Azide free (Capture) ab267423
- Anti-Endostatin antibody [EPR22479-286] - BSA and Azide free (Detector) ab267424
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 115 %", "average": "= 106" }, { "sample": "Serum", "range": "94 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "94 - 102 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "86 - 96 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "101 - 105 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (PubMed : 9008168). Inhibits VEGFA isoform VEGF165-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2 (PubMed : 12029087). Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (PubMed : 11158588). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (By similarity).
See full target information Col18a1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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