AB230938

Mouse eNOS ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Mouse eNOS ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse eNOS with a sensitivity of 5.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ecnos, Nos3, Nitric oxide synthase 3, Constitutive NOS, EC-NOS, NOS type III, cNOS, NOSIII, Endothelial NOS, eNOS

2 Images

Sandwich ELISA - Mouse eNOS ELISA Kit (AB230938)

sELISA

Supplier Data

Sandwich ELISA - Mouse eNOS ELISA Kit (AB230938)

Interpolated concentrations of native eNOS in mouse kidney extract, heart extract, brain extract, and bEnd.3 cell extract samples.

The concentrations of eNOS were measured in duplicates, interpolated from the eNOS standard curves and corrected for sample dilution. Undiluted samples are as follows : kidney extract 1,000 μg/mL, heart extract 1,000 μg/mL, brain extract 250 μg/mL, and bEnd.3 extract 25 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean eNOS concentration in the undiluted samples was determined to be 1,259 pg/mL in kidney extract, 2,998 pg/mL in heart extract, 194 pg/mL in brain extract, and 1,419 pg/mL in bEnd.3 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse eNOS ELISA Kit (AB230938)

Example of mouse eNOS standard curve in 1X Cell Extraction Buffer PTR.

The eNOS standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Mouse brain extract, Mouse muscle extract

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 5.58 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse eNOS ELISA Kit (ab230938) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of eNOS protein in tissue extracts, mouse brain extract, mouse muscle extract, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse eNOS with 5.58 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "bEnd.3 cells", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.04" }, { "reproducibilityType": "Intra", "sample": "bEnd.3 cells", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]

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Recovery

[ { "sample": "Mouse muscle extract", "range": "102 - 108 %", "average": "= 106" }, { "sample": "Cell culture extracts", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Tissue Extracts", "range": "95 - 98 %", "average": "= 96" }, { "sample": "Mouse brain extract", "range": "95 - 97 %", "average": "= 96" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab230938-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB230938-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB230938-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB230938-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB230938-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse eNOS Lyophilized Recombinant Protein", "number":"AB230938-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse eNOS Capture Antibody", "number":"AB230938-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB230938-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB230938-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse eNOS Detector Antibody", "number":"AB230938-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB230938-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB230938-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ENOS also known as endothelial nitric oxide synthase is an enzyme important for the production of nitric oxide (NO) in blood vessels. This protein with a molecular weight of approximately 133 kDa is expressed mostly in endothelial cells. eNOS plays a mechanical role in synthesizing NO from L-arginine a process requiring cofactors such as NADPH and oxygen. The activity of eNOS can be investigated through techniques such as Western blot with specific assays like phospho-eNOS ELISA available to measure its phosphorylated forms indicating activated states of the enzyme.

Biological function summary

ENOS contributes significantly to the regulation of vascular tone and blood flow by promoting vasodilation. It does not function alone; instead it forms complexes with other proteins to exert its full effect. eNOS activity influences the process of angiogenesis inflammation modulation and platelet aggregation. Through its ability to produce nitric oxide eNOS acts as a signaling molecule helping maintain vascular homeostasis.

Pathways

ENOS is a critical player in the NO signaling pathway and interacts intricately with the PI3K/Akt pathway. Nitric oxide produced by eNOS has a role in signaling cascades that lead to vascular dilation and reduced blood pressure. The PI3K/Akt pathway regulates eNOS activity via phosphorylation enhancing NO production. Other proteins like caveolin-1 and calmodulin modulate eNOS impacting these pathways' outcomes.

ENOS is associated with cardiovascular conditions like atherosclerosis and hypertension. Dysfunction of eNOS can lead to reduced NO production impairing vasodilation and contributing to these diseases. In cardiovascular disorders eNOS interacts with proteins such as the angiotensin II type 1 receptor which can negatively impact its function exacerbating disease states. Investigating eNOS and its related proteins provides insight into potential therapeutic targets for improving cardiovascular health.

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Product protocols

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Target data

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.

See full target information Nos3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Food science & nutrition 12:7544-7551 PubMed39479634

2024

Anti-dyslipidemic effects of root and (.) Merrill extracts fermented with in ovariectomized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Min Jang,Jimyeong Ha,Insuk Choi

Research (Washington, D.C.) 7:0331 PubMed38550779

2024

DNA-PKcs Phosphorylates Cofilin2 to Induce Endothelial Dysfunction and Microcirculatory Disorder in Endotoxemic Cardiomyopathy.

Applications

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Species

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Yingzhen Du,Pingjun Zhu,Yukun Li,Jiachi Yu,Tian Xia,Xing Chang,Hang Zhu,Ruibing Li,Qingyong He

Brain : a journal of neurology 145:1449-1463 PubMed35048960

2022

VEGF signalling causes stalls in brain capillaries and reduces cerebral blood flow in Alzheimer's mice.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Ali,Kaja Falkenhain,Brendah N Njiru,Muhammad Murtaza-Ali,Nancy E Ruiz-Uribe,Mohammad Haft-Javaherian,Stall Catchers,Nozomi Nishimura,Chris B Schaffer,Oliver Bracko

Biomedicines 8: PubMed33322134

2020

The Inhibition of Prolyl Oligopeptidase as New Target to Counteract Chronic Venous Insufficiency: Findings in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Casili,Marika Lanza,Sarah Adriana Scuderi,Salvatore Messina,Irene Paterniti,Michela Campolo,Emanuela Esposito

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse eNOS ELISA Kit

Sandwich ELISA - Mouse eNOS ELISA Kit (AB230938)

Sandwich ELISA - Mouse eNOS ELISA Kit (AB230938)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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