Mouse Eotaxin-2 ELISA Kit
- Recombinant
- SimpleStep
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(2 Publications)
Mouse Eotaxin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Eotaxin-2 with a sensitivity of 6.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Scya24, Ccl24, C-C motif chemokine 24, Eosinophil chemotactic protein 2, Small-inducible cytokine A24, Eotaxin-2
- sELISA
Supplier Data
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (AB206985)
Linearity of dilution of mouse Eotaxin-2 in cell culture media and stimulated mouse lung supernatants.
Recombinant mouse Eotaxin-2 was spiked into 1 : 4 cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. Native mouse Eotaxin-2 was measured in 1 : 20 stimulated mouse lung supernatants which was further diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Eotaxin-2 were measured in duplicate and interpolated from the mouse Eotaxin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (AB206985)
Assay specificity is demonstrated on mouse lung supernatants.
Mouse lung supernatants were cultured in cell culture media with 10% fetal bovine serum for 3 days in the absence (unstimulated) and presence (stimulated) of 10 ng/mL of human IL-4. Both unstimulated and stimulated mouse lung supernatants were measured in duplicate at a 1 : 20 dilution. The Raw O.D. values for each sample are graphed, with the background O.D. shown as the dashed line. The stimulated mouse lung supernatants diluted 1 : 20 measured at 1,207 pg/mL, whereas the unstimulated mouse lung supernatants diluted 1 : 20 measured below the limit of detection of the assay.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (AB206985)
Native linearity of dilution of mouse Eotaxin-2 in normal serum and normal plasma samples.
Native mouse Eotaxin-2 was measured in neat normal serum, neat normal citrate plasma, and neat normal EDTA plasma diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Eotaxin-2 were measured in duplicate and interpolated from the mouse Eotaxin 2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). For reference, 8X or more diluted normal serum and normal citrate plasma samples interpolate below the standard curve. 16X or more diluted Normal EDTA plasma samples interpolate below the standard curve.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (AB206985)
Spiked linearity of dilution of mouse Eotaxin-2 in normal serum and normal plasma samples.
Recombinant mouse Eotaxin-2 was spiked into neat serum, neat citrate plasma, and neat EDTA plasma diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Eotaxin-2 were measured in duplicate and interpolated from the mouse Eotaxin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
- sELISA
Supplier Data
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (AB206985)
Example of the mouse Eotaxin-2 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity data
Product details
Mouse Eotaxin-2 ELISA Kit (ab206985) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin-2 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Eotaxin-2 with 6.23 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Eotaxin-2, also known as CCL24, MPIF-2 or Ckβ-6, is a C-C chemokine originally identified in activated monocytes. Eotaxin-2 is expressed in activated T lymphocytes, GM-CSF treated macrophages and dermal fibroblasts. Mouse eotaxin-2 is a highly glycosylated protein which requires maturation by removal of a 26 aminoacid signal peptide prior to secretion. Secretion of Eotaxin-2 is known to recruit and activate CCR3-bearing cells particularly eosinophils as well as basophils. Activation of eosinophils generates reactive oxygen species, lipid mediators of inflammation and degranulation of toxic granule proteins. Chemotaxis and activation of basophils, previously primed with IL-3, lead to release of histamine and leukotriene C4.
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Properties and storage information
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Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Eotaxin 2 plays a critical role in the immune response by attracting eosinophils and other immune cells to inflamed or damaged tissues. This attraction is mediated through its interaction with the CCR3 receptor on the surface of target cells. Eotaxin-2 is not part of a larger complex but instead acts as a standalone molecule within the chemokine signaling network. This protein assists in the body's defense by guiding the movement of cells to stimulate immune activity where needed.
Pathways
Eotaxin 2 is involved in the chemokine-mediated signaling pathway that directs the migration of immune cells. Another important pathway associated with eotaxin 2 is the inflammatory response pathway. The coordinated interaction with other chemokines such as eotaxin (CCL11) and MCP-4 (CCL13) is essential to its role in inflammation and healing. These interactions facilitate proper cellular responses to various physiological challenges.
Product protocols
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Publications (2)
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Chemical biology & drug design 105:e70142 PubMed40556500
2025
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The Journal of investigative dermatology 139:1957-1965.e2 PubMed30910757
2019
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