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AB100681

Mouse Eotaxin-2 ELISA Kit (CCL24)

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(1 Publication)

Mouse Eotaxin-2 ELISA Kit (CCL24) is a Sandwich ELISA for the measurement of Mouse Eotaxin-2 (CCL24) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Scya24, Ccl24, C-C motif chemokine 24, Eosinophil chemotactic protein 2, Small-inducible cytokine A24, Eotaxin-2

2 Images
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (CCL24) (AB100681)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (CCL24) (AB100681)

Representative Standard Curve using ab100681

Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (CCL24) (AB100681)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (CCL24) (AB100681)

Representative Standard Curve using ab100681

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 4 pg/mL

Range

3.28 - 800 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Eotaxin-2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Eotaxin-2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse Eotaxin-2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Eotaxin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Eotaxin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 102 %", "average": "= 96.65" }, { "sample": "Serum", "range": "93 - 112 %", "average": "= 103.1" }, { "sample": "Plasma", "range": "79 - 137 %", "average": "= 113.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100681-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100681-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100681-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100681-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Eotaxin-2 Microplate (12 x 8 wells)", "number":"AB100681-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Eotaxin-2", "number":"AB100681-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100681-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Eotaxin-2 Standard (lyophilized)", "number":"AB100681-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100681-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100681-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin 2 also known as CCL24 or eotaxin-2 is a chemokine protein with a mass of approximately 10 kDa. It mainly functions as a signaling molecule that recruits specific immune cells to sites of inflammation or tissue damage. This protein is expressed in various tissues with higher levels in the heart lung liver and small intestine. Eotaxin-2 is a member of the CC chemokine family and its expression is often regulated by inflammatory cytokines.
Biological function summary

Eotaxin 2 plays a critical role in the immune response by attracting eosinophils and other immune cells to inflamed or damaged tissues. This attraction is mediated through its interaction with the CCR3 receptor on the surface of target cells. Eotaxin-2 is not part of a larger complex but instead acts as a standalone molecule within the chemokine signaling network. This protein assists in the body's defense by guiding the movement of cells to stimulate immune activity where needed.

Pathways

Eotaxin 2 is involved in the chemokine-mediated signaling pathway that directs the migration of immune cells. Another important pathway associated with eotaxin 2 is the inflammatory response pathway. The coordinated interaction with other chemokines such as eotaxin (CCL11) and MCP-4 (CCL13) is essential to its role in inflammation and healing. These interactions facilitate proper cellular responses to various physiological challenges.

Eotaxin 2 has been implicated in asthma and allergic rhinitis conditions characterized by an overactive immune response. In these disorders its production and release contribute to excessive eosinophil accumulation which can exacerbate symptoms. Eotaxin 2 connects to these diseases primarily through its action on the CCR3 receptor and its interaction with related chemokines such as eotaxin (CCL11) which also play a role in promoting eosinophil recruitment and activation.
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Product protocols

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Target data

Chemotactic for resting T-lymphocytes, and eosinophils (PubMed : 15647285). Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3 (By similarity).
See full target information Ccl24
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:7471 PubMed28785009

2017

Commensal microbiota maintains alveolar macrophages with a low level of CCL24 production to generate anti-metastatic tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Min Cheng,Yongyan Chen,Liang Wang,Wen Chen,Ling Yang,Guodong Shen,Tingjuan Xu,Gan Shen,Zhigang Tian,Shilian Hu
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Product promise

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