Mouse Eotaxin-2 ELISA Kit (CCL24) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Eotaxin-2 (CCL24) in Mouse in Plasma, Cell culture supernatant, Serum samples.
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- Scientific reports 7:74712017Commensal microbiota maintains alveolar macrophages with a low level of CCL24 production to generate anti-metastatic tumor activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Cheng et. al.PubMed 28785009
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 3.28 - 800 pg/mL
- Sensitivity
- < 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemotactic for resting T-lymphocytes, and eosinophils (PubMed:15647285). Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3 (By similarity).
Alternative names
Scya24, Ccl24, C-C motif chemokine 24, Eosinophil chemotactic protein 2, Small-inducible cytokine A24, Eotaxin-2
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Mouse Eotaxin-2 ELISA Kit (CCL24) is a Sandwich (quantitative) ELISA kit for the measurement of Mouse Eotaxin-2 (CCL24) in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 3.28 - 800 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 4 pg/mL
- General recovery
- 100%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 96.65 | Range 89 - 102 % |
Sample type Serum | Average % = 103.1 | Range 93 - 112 % |
Sample type Plasma | Average % = 113.1 | Range 79 - 137 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Eotaxin-2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Eotaxin-2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Eotaxin-2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Eotaxin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Eotaxin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Eotaxin 2 also known as CCL24 or eotaxin-2 is a chemokine protein with a mass of approximately 10 kDa. It mainly functions as a signaling molecule that recruits specific immune cells to sites of inflammation or tissue damage. This protein is expressed in various tissues with higher levels in the heart lung liver and small intestine. Eotaxin-2 is a member of the CC chemokine family and its expression is often regulated by inflammatory cytokines.
- Biological function summary
Eotaxin 2 plays a critical role in the immune response by attracting eosinophils and other immune cells to inflamed or damaged tissues. This attraction is mediated through its interaction with the CCR3 receptor on the surface of target cells. Eotaxin-2 is not part of a larger complex but instead acts as a standalone molecule within the chemokine signaling network. This protein assists in the body's defense by guiding the movement of cells to stimulate immune activity where needed.
- Pathways
Eotaxin 2 is involved in the chemokine-mediated signaling pathway that directs the migration of immune cells. Another important pathway associated with eotaxin 2 is the inflammatory response pathway. The coordinated interaction with other chemokines such as eotaxin (CCL11) and MCP-4 (CCL13) is essential to its role in inflammation and healing. These interactions facilitate proper cellular responses to various physiological challenges.
- Associated diseases and disorders
Eotaxin 2 has been implicated in asthma and allergic rhinitis conditions characterized by an overactive immune response. In these disorders its production and release contribute to excessive eosinophil accumulation which can exacerbate symptoms. Eotaxin 2 connects to these diseases primarily through its action on the CCR3 receptor and its interaction with related chemokines such as eotaxin (CCL11) which also play a role in promoting eosinophil recruitment and activation.
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2 product images
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (CCL24) (ab100681)
Representative Standard Curve using ab100681
Sandwich ELISA - Mouse Eotaxin-2 ELISA Kit (CCL24) (ab100681)
Representative Standard Curve using ab100681
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