AB201277

Mouse Eotaxin ELISA Kit (CCL11)

Recombinant
SimpleStep
What is this?

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(2 Publications)

Mouse Eotaxin ELISA Kit (CCL11) is a single-wash 90-min Simplestep used to quantify Mouse Eotaxin (CCL11) with a sensitivity of 8.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Scya11, Ccl11, Eotaxin, C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11

2 Images

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

sELISA

Supplier Data

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Linearity of dilution of the assay. Interpolated concentrations of Eotaxin in mouse serum, plasma (EDTA) and culture media.

The concentrations of Eotaxin were measured in duplicate and interpolated from the Eotaxin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin concentration was determined to be 484 pg/mL in mouse serum and 668 pg/mL in mouse plasma.

sELISA

Supplier Data

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Example of Eotaxin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.1 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Eotaxin ELISA Kit (CCL11) (ab201277) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin (CCL11) protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Eotaxin (CCL11) with 8.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse Eotaxin (CCL11) is a 8.4 kDa cytokine, constitutively expressed in the thymus and inducibly expressed in the lung, intestine, heart, spleen and kidney. Eotaxin is a chemoattractant for eosinophils during inflammation and allergic reactions, and is also involved in mast cell differentiation and growth of myeloid cell progenitors.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 96 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "99 - 107 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "99 - 100 %", "average": "= 99" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

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Product protocols

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Target data

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells (PubMed : 7568052, PubMed : 8574847). Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.

See full target information Ccl11

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 50:75-83 PubMed36086967

2022

Zeaxanthin attenuates OVA-induced allergic asthma in mice by regulating the p38 MAPK/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosheng Jin,Weijing Jin,Guoping Li,Jisheng Zheng

Scientific reports 7:5334 PubMed28706221

2017

CCL11, a novel mediator of inflammatory bone resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Elin Kindstedt,Cecilia Koskinen Holm,Rima Sulniute,Irene Martinez-Carrasco,Richard Lundmark,Pernilla Lundberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Eotaxin ELISA Kit (CCL11)

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (AB201277)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise