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AB100682

Mouse Epiregulin ELISA Kit (EREG)

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Mouse Epiregulin ELISA Kit (EREG) is a Sandwich ELISA for the measurement of Mouse Epiregulin (EREG) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Proepiregulin, Ereg

2 Images
Sandwich ELISA - Mouse Epiregulin ELISA Kit (EREG) (AB100682)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Epiregulin ELISA Kit (EREG) (AB100682)

Representative Standard Curve using ab100682

Sandwich ELISA - Mouse Epiregulin ELISA Kit (EREG) (AB100682)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Epiregulin ELISA Kit (EREG) (AB100682)

Representative Standard Curve using ab100682

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Complementary Products

Assay Kits

AB100668

Mouse Amphiregulin ELISA Kit

Sandwich ELISA - Mouse Amphiregulin ELISA Kit (AB100668)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB233512

Anti-EREG antibody

Western blot - Anti-EREG antibody (AB233512)

  • 0
  • 0
Assay Kits

AB100696

Mouse IGF2 ELISA Kit

ELISA - Mouse IGF2 ELISA Kit (AB100696)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Mouse Epiregulin in normal serum with ab100682, only in serum spiked with Mouse Epiregulin.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 110 %", "average": "= 102.6" }, { "sample": "Plasma", "range": "67 - 90 %", "average": "= 73.94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100682-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100682-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100682-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Epiregulin Microplate (12 x 8 wells)", "number":"AB100682-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100682-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100682-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100682-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100682-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Epiregulin", "number":"AB100682-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Epiregulin Standard (lyophilized)", "number":"AB100682-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epiregulin also known as EREG is a member of the epidermal growth factor (EGF) family. It is a glycoprotein with a molecular weight of approximately 19 kDa. The protein is secreted and functions as a ligand for the EGF receptor (EGFR) and to a lesser extent for ErbB4 known as HER4 in humans. Epiregulin is expressed in a variety of tissues including placenta kidney and skin as well as within certain tumor cells. The expression can be regulated by signaling pathways involved in cellular growth and differentiation.
Biological function summary

Epiregulin participates in cellular processes such as proliferation differentiation and migration. It functions by binding to the EGFR initiating a cascade of downstream signaling events. As part of the EGF family it does not form part of a larger complex but it influences the activity of receptor dimers once bound. Its activity varies depending on the cellular context aiding in processes like tissue repair and embryonic development through its interaction with receptors and activation of signaling pathways.

Pathways

Epiregulin plays a significant role in the MAPK/ERK and PI3K/Akt signaling pathways both critical in regulating cell growth and survival. These pathways influence critical processes like cell cycle progression and apoptosis where epiregulin functions in coordination with other EGF family proteins such as TGF-alpha and amphiregulin. This protein assists in maintaining cellular homeostasis by modulating these pathways which are key to responding to environmental stimuli and cellular stress.

Epiregulin has associations with certain cancers such as colorectal and breast cancer. The protein often shows elevated expression levels in tumor samples implicating it in tumorigenesis. Its binding interaction with EGFR plays an important role in cancer cell survival and proliferation. Additionally epiregulin bears a connection to inflammatory diseases where its expression can exacerbate conditions like psoriasis through its impact on epidermal cells. In both cancer and inflammatory conditions the interplay between epiregulin and other related proteins like EGFR and ERBB2 influences disease progression and severity.
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Product protocols

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Target data

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (By similarity). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed : 24631357).
See full target information Ereg
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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