AB270893

Mouse Erythropoietin ELISA Kit (EPO)

Recombinant
SimpleStep
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Mouse Erythropoietin ELISA Kit (EPO) is a single-wash 90-min Simplestep used to quantify Mouse Erythropoietin (EPO) with a sensitivity of 2.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Erythropoietin, Epo

5 Images

ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

ELISA

Supplier Data

ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

sELISA

Supplier Data

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Interpolated concentrations of native Erythropoietin in mouse C57BL/6, CD-1 and Balb/C serum samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : C57BL/6 serum 100%, CD-1 serum 100% and Balb/C serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 478 pg/mL in C57BL/6 serum, 66 pg/mL in CD-1 serum and 68 pg/mL in Balb/C serum.

sELISA

Supplier Data

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Mouse Erythropoietin competitor curve comparison.

Standard Curve comparison between Mouse Erythropoiethin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Example of mouse Erythropoietin standard curve in Sample Diluent NS.

The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Interpolated concentrations of native Erythropoietin in mouse CD-1 plasma samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 95 pg/mL in plasma (citrate), 36 pg/mL in plasma (EDTA) and 153 pg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 2.35 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Erythropoietin ELISA Kit (EPO) ab270893 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Erythropoietin (EPO)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.35 pg/mL.

How the assay works

Mouse Erythropoietin (EPO)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Erythropoietin ELISA Kit (EPO) ab270893 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Mouse Erythropoietin has 94% amino acids sequence identity to rat Erythropoietin, and 80% identity to the human Erythropoietin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 112 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "98 - 101 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "99 - 101 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "89 - 104 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "91 - 98 %", "average": "= 95" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab270893-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB270893-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270893-CMP08" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB270893-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270893-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB270893-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270893-CMP03" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB270893-CMP06" }, { "size":"20 x 1 Vial", "name":"Mouse Erythropoietin (EPO) Lyophilized Recombinant Protein", "number":"AB270893-CMP02" }, { "size":"10 x 600 µL", "name":"10X Mouse Erythropoietin (EPO) Capture Antibody", "number":"AB270893-CMP09" }, { "size":"10 x 600 µL", "name":"10X Mouse Erythropoietin (EPO) Detector Antibody", "number":"AB270893-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270893-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270893-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270893-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270893-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB270893-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270893-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270893-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Erythropoietin (EPO) Detector Antibody", "number":"AB270893-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270893-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Erythropoietin (EPO) Lyophilized Recombinant Protein", "number":"AB270893-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Erythropoietin (EPO) Capture Antibody", "number":"AB270893-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.

Biological function summary

Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.

Pathways

Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.

Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.

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Product protocols

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Target data

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

See full target information Erythropoietin

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111072 PubMed35830790

2022

Autism-associated chromatin remodeler CHD8 regulates erythroblast cytokinesis and fine-tunes the balance of Rho GTPase signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowei Tu,Cuiqing Fan,Ashely K Davis,Mengwen Hu,Chen Wang,Akhila Dandamudi,Katie G Seu,Theodosia A Kalfa,Q Richard Lu,Yi Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Erythropoietin ELISA Kit (EPO)

ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Sandwich ELISA - Mouse Erythropoietin ELISA Kit (EPO) (AB270893)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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