AB285291

Mouse Estrogen ELISA Kit

Name: Mouse Estrogen ELISA Kit (ab285291) | Abcam
Brand: Abcam Limited
SKU: AB285291

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(4 Publications)

Mouse Estrogen ELISA Kit is a Competitive ELISA used to quantify Mouse Estrogen with a sensitivity of 9.375 pg/ml

- Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader
- Wide dynamic range – 15.625 - 1000 pg/ml

View Alternative Names

Atherosclerosis, susceptibility to, included, DKFZp686N23123, ER, ER-alpha, ER-beta, ER[a], ER[b], ESR, ESR B, ESR BETA, ESR1_HUMAN, ESRA, ESTR B, Era, Erb, Erb2, Estr, Estra, Estradiol Receptor alpha, Estradiol Receptor beta, Estradiol receptor, Estrogen Receptor 1, Estrogen Receptor 2, Estrogen receptor, Estrogen receptor 1 (alpha), Estrogen receptor 2 (ER beta), Estrogen receptor 2 ER beta, Estrogen receptor alpha, Estrogen receptor beta 4, Estrogen resistance, included, HDL cholesterol, augmented response of, to hormone replacement, included, Myocardial infarction, susceptibility to, included, NR3A1, NR3A2, Nuclear receptor subfamily 3 group A member 1, Nuclear receptor subfamily 3 group A member 2, OTTHUMP00000017718, OTTHUMP00000017719, RNESTROR

1 Images

Competitive ELISA - Mouse Estrogen ELISA Kit (AB285291)

cELISA

Supplier Data

Competitive ELISA - Mouse Estrogen ELISA Kit (AB285291)

Standard Estrogen Curve.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Assay type

Quantitative

Sensitivity

< 9.375 pg/mL

Range

15.625 - 1000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Estrogen ELISA Kit is a competitive ELISA kit for the quantitative measurement of mouse estrogen with a sensitivity of 9.375 pg/mL

How the assay works

Mouse Estrogen ELISA kit an285291 is a competitive ELISA kit.

There are 2 types of competitive ELISAs:
- "antigen down": The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
- "antibody down": the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Mouse Estrogen ELISA kit ab285291 protocol summary

1. wash plate before adding standards, controls and samples
2. Add standard, samples and controls into appropriate wells. Incubate 45min
3. Wash
4. Add SABC solution into each well. Incubate for 30 min
5. Wash
6. Add TMB substrate into each well. Incubate for 15-30 min
7. Add stop solution. Read at 450 nm within 20 min.

How other researchers are using Mouse Estrogen ELISA kit ab285291

Mouse estrogen ELISA kit has been used to study the role of mitochondria dynamics in the female reproductive system in mice⁽¹⁾ and to study the mechanism of the NKB-NK3R pathway in Polycystic ovary syndrome (PCOS).⁽²⁾

References
⁽¹⁾ YP Budi et al. PMID 36765080
⁽²⁾Fei Guo et al. PMID 36453600

Related and Recommended products

Mouse estrogen ELISA kit ab285291 is commonly used to study the reproductive system in mice. Another kit to assess reproductive hormones in mice is
- Mouse/Rat testosterone ELISA kit ab285350

This product is manufactured by BioVision, an Abcam company and was previously called K4265 Estrogen (Mouse) ELISA Kit. K4265-100 is the same size as the 96 test size of ab285291.

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Precision

[ { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

See full target information Estradiol

Additional targets

Estrone,

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Basic and clinical andrology 35:9 PubMed40055615

2025

Pelvic vasectomy and its protective effects on rat testis function.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Yang,Yujun Chen,Xiaofeng Cheng,Jingxin Wu,Ruohui Huang,Biao Qian,Gongxian Wang

Scientific reports 13:2392 PubMed36765080

2023

The injections of mitochondrial fusion promoter M1 during proestrus disrupt the progesterone secretion and the estrous cycle in the mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Yovita Permata Budi,Meng-Chieh Hsu,Yi-Chun Lin,Yue-Jia Lee,Hsin-Yi Chiu,Chih-Hsien Chiu,Yi-Fan Jiang

American journal of reproductive immunology (New York, N.Y. : 1989) 89:e13663 PubMed36453600

2022

The overexpression of neurokinin B-neurokinin 3 receptor system exerts direct effects on the ovary under PCOS-like conditions to interfere with mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Taniya Fernando,Xiaoyong Zhu,Yingli Shi

American journal of physiology. Heart and circulatory physiology 323:H941-H948 PubMed36206053

2022

Ovarian hormones do not mediate protection against pulmonary hypertension and right ventricular remodeling in female mice exposed to chronic, inhaled nicotine.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas D Fried,Anna Whitehead,Eric Lazartigues,Xinping Yue,Jason D Gardner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Estrogen ELISA Kit

Competitive ELISA - Mouse Estrogen ELISA Kit (AB285291)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (4)

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Secondary Antibodies

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Product promise