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AB277426

Mouse FABP4 ELISA

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(1 Publication)

Mouse FABP4 ELISA is a Sandwich (quantitative) ELISA for the measurement of Mouse FABP4 ELISA in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Ap2, Fabp4, 3T3-L1 lipid-binding protein, Adipocyte lipid-binding protein, Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Myelin P2 protein homolog, P15, P2 adipocyte protein, Protein 422, ALBP, A-FABP, AFABP

1 Images
ELISA - Mouse FABP4 ELISA (AB277426)
  • ELISA

Supplier Data

ELISA - Mouse FABP4 ELISA (AB277426)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 14 pg/mL

Range

12.29 - 3000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Mouse FABP4 ELISA Kit (ab277426) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FABP4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse FABP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse FABP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse FABP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse FABP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "69 - 80 %", "average": "= 72.64" }, { "sample": "Plasma", "range": "67 - 79 %", "average": "= 72.89" }, { "sample": "Cell culture media", "range": "128 - 139 %", "average": "= 133.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277426-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277426-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277426-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277426-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse FABP4 coated Microplate", "number":"AB277426-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse FABP4 standard protein (Lyophilized)", "number":"AB277426-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB277426-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse FABP4 Antibody", "number":"AB277426-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277426-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid-binding protein 4 (FABP4) also known as aP2 is a member of the fatty acid-binding protein family. It predominantly exists in adipocytes and macrophages serving an important role in lipid metabolism and transport. FABP4 facilitates the binding and transport of long-chain fatty acids and other hydrophobic molecules within cells. The molecular weight of FABP4 is approximately 14-15 kDa. It exhibits high expression levels in adipose tissue and is inducible in macrophages under certain conditions.
Biological function summary

This protein participates in the regulation of lipid homeostasis and energy metabolism. FABP4 is part of the lipid-binding protein family that helps maintain fatty acid equilibrium within cells. By controlling the transport and signaling of lipids FABP4 impacts lipid storage insulin sensitivity and inflammation processes. Its activity is not confined to a specific multimeric complex yet it interacts with other proteins to exert its functions contributing to significant metabolic pathways in the body.

Pathways

FABP4 plays essential roles in the regulation of metabolic and inflammatory signaling pathways. It is intricately involved in the peroxisome proliferator-activated receptor gamma (PPARγ) pathway where it modulates adipose tissue development and glucose metabolism. In addition FABP4 has connections with the c-Jun N-terminal kinase (JNK) signaling pathway influencing stress and inflammatory responses. Interactions with these pathways place FABP4 at a link with proteins like PPARγ and JNK essential for cellular metabolic balance and responses to metabolic stress.

Researchers connect FABP4 to conditions such as obesity and type 2 diabetes. Its expression levels correlate with insulin resistance and metabolic syndrome making it a target of interest in these metabolic disorders. FABP4 interacts with other proteins like adiponectin which is important in modulating glucose levels and fatty acid breakdown. Understanding FABP4's role in these pathways can facilitate the development of therapeutic strategies aimed at targeting metabolic dysfunctions associated with obesity and diabetes.
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Product protocols

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Target data

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
See full target information Fabp4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:10540-10551 PubMed35441580

2022

Downregulation of fatty acid binding protein 4 alleviates lipid peroxidation and oxidative stress in diabetic retinopathy by regulating peroxisome proliferator-activated receptor γ-mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao'e Fan,Manhong Xu,Qianfeng Ren,Yan Fan,Boshi Liu,Jiaqi Chen,Zhengmin Wang,Xiaoyan Sun
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