Mouse Factor IX/PTC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Factor IX/PTC in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 375 - 12000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 97.19 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Alternative names
Cf9, F9, Coagulation factor IX, Christmas factor
What's included?
Mouse Factor IX/PTC ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse Factor IX/PTC in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 375 - 12000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 97.19 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 0.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 100 | Range 89 - 114 % |
Sample type Citrate plasma | Average % = 99 | Range 91 - 107 % |
Sample type EDTA Plasma | Average % = 98 | Range 81 - 109 % |
Sample type Heparin Plasma | Average % = 114 | Range 106 - 122 % |
Sample type Cell culture media | Average % = 94 | Range 87 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Factor IX/PTC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Mouse Factor IX/PTC with 97.19 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (ab323929)
Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:250 mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (ab323929)
Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are 1:250 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (ab323929)
Example of mouse Factor IX/PTC standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (ab323929)
Example of mouse Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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