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AB323929

Mouse Factor IX/PTC ELISA Kit

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Mouse Factor IX/PTC ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Factor IX/PTC in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Cf9, F9, Coagulation factor IX, Christmas factor

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Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 250 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)

Example of mouse Factor IX/PTC standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 250 mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Factor IX/PTC ELISA Kit (AB323929)

Example of mouse Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 97.19 pg/mL

Range

375 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Factor IX/PTC SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor IX/PTC protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Mouse Factor IX/PTC with 97.19 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 0.5" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 114 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "91 - 107 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "81 - 109 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "106 - 122 %", "average": "= 114" }, { "sample": "Cell culture media", "range": "87 - 102 %", "average": "= 94" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
See full target information F9
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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