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Mouse Ferritin ELISA Kit (FTL) ab157713 is used to quantify Mouse Ferritin with a sensitivity of 64 pg/ml.

Sandwich ELISA Kit


Images

Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (AB157713), expandable thumbnail
  • Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (AB157713), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Serum, Other biological fluids
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
12.5 - 400 ng/mL
Sensitivity
= 3.1631 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Degraded to release iron upon autophagy activation by nutrient starvation (PubMed:25327288).

Alternative names

What's included?

1 x 96 Tests
Components
100X HRP-conjugated anti-mouse Ferritin antibody
1 x 150 µL
20X Wash Buffer Concentrate
1 x 50 mL
5X Diluent Concentrate
1 x 50 mL
Chromogen Substrate Solution
1 x 12 mL
Mouse Ferritin Calibrator (lyophilized)
1 x 1 Vial
Mouse Ferritin ELISA Microplate
1 x 1 Unit
Stop Solution
1 x 12 mL

Recommended products

Mouse Ferritin ELISA Kit (FTL) ab157713 is used to quantify Mouse Ferritin with a sensitivity of 64 pg/ml.

Sandwich ELISA Kit

Key facts

Detection method
Colorimetric
Sample types
Plasma, Serum, Other biological fluids
Assay type
Sandwich (quantitative)
Reactive species
Mouse
Range
12.5 - 400 ng/mL
Assay Platform
Microplate
Sensitivity
= 3.1631 ng/mL

Precision

Intra assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 10

Inter assay

Sample
Overall
n
0
mean
-
SD
-
C.V.
< 10

Recovery

Sample specific recovery

Sample type
Serum
Average %
> 85
Range

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Mouse Ferritin ELISA kit (FTL) is a highly sensitive ELISA designed for the quantitation of Ferritin (FTL) in mouse biological samples.

In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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2 product images

  • Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (ab157713), expandable thumbnail

    Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (ab157713)

    Ferritin measured in biologicals showing quantity (ng) per mL of tested sample

  • Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (ab157713), expandable thumbnail

    Sandwich ELISA - Mouse Ferritin ELISA Kit (FTL) (ab157713)

    Representative standard curve using ab157713 Ferritin Mouse ELISA kit

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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