Mouse Ferritin ELISA Kit (FTL) ab157713 is used to quantify Mouse Ferritin with a sensitivity of 64 pg/ml.
Sandwich ELISA Kit
Application | Reactivity | Dilution info | Notes |
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Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Degraded to release iron upon autophagy activation by nutrient starvation (PubMed:25327288).
Ftl, Ftl-1, Ftl1, Ferritin light chain 1, Ferritin L subunit 1
Mouse Ferritin ELISA Kit (FTL) ab157713 is used to quantify Mouse Ferritin with a sensitivity of 64 pg/ml.
Sandwich ELISA Kit
Sample | n | mean | SD | C.V. |
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Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample | n | mean | SD | C.V. |
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Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Sample type | Average % | Range |
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Sample type Serum | Average % > 85 | Range |
Mouse Ferritin ELISA kit (FTL) is a highly sensitive ELISA designed for the quantitation of Ferritin (FTL) in mouse biological samples.
In this assay the Ferritin present in samples reacts with the anti-Ferritin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Ferritin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Ferritin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Ferritin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Ferritin in the test sample. The quantity of Ferritin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Ferritin measured in biologicals showing quantity (ng) per mL of tested sample
Representative standard curve using ab157713 Ferritin Mouse ELISA kit
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