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AB212160

Mouse FGF-21 ELISA Kit

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(21 Publications)

Mouse FGF-21 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse FGF-21 with a sensitivity of 1.47 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 20 citations

View Alternative Names

Fibroblast growth factor 21, FGF-21, Fgf21

8 Images
ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • ELISA

Supplier Data

ELISA - Mouse FGF-21 ELISA Kit (AB212160)
Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Example of mouse FGF-21 standard curve in Sample Diluent NS. The FGF-21 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Example of mouse FGF-21 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Interpolated concentrations of native FGF-21 in mouse serum and plasma samples.

The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor (to neat sample) corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 409 pg/mL in neat serum, 397 pg/mL in neat plasma (citrate), 358 pg/mL in neat plasma (EDTA), and 348 pg/mL in neat plasma (heparin).

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Interpolated concentrations of native FGF-21 in untreated L-929 cell culture supernatant samples.

The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : L-929 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 59 pg/mL in neat L-929 cell culture supernatant.

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native FGF-21 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Unknown

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Mouse FGF-21 standard curve comparison data.

Standard curve comparison between mouse FGF-21 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 8-fold increase in sensitivity.

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit (AB212160)

Interpolated concentrations of FGF-21 in normal female CD-1 mouse serum and pregnant female CD-1 mouse serum at day 18 of gestation.

The concentrations of FGF-21 were measured in three different dilutions in duplicate and interpolated from the FGF-21 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in pg FGF-21 per mL of neat serum (mean +/- SD, n=3). FGF-21 concentration was determined to be 401 pg/mg in normal mouse serum, and 9,193 pg/mL in pregnant mouse serum.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.47 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse FGF-21 ELISA Kit ab212160 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse FGF-21 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.47 pg/mL.

How the assay works

Mouse FGF-21 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse FGF-21 ELISA Kit ab212160 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-FGF21 antibody [EPR18010-51] - BSA and Azide free (Capture) ab242811
- Anti-FGF21 antibody [EPR18010-48] - BSA and Azide free (Detector) ab242811

FGF-21 is a secreted protein, a member of a large FGF gene family. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression but not via SLC2A4/GLUT4 expression. FGF-21 activity probably requires the presence of KLB.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 97 %", "average": "= 94" }, { "sample": "Serum", "range": "99 - 107 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "92 - 108 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "88 - 104 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "94 - 106 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
Biological function summary

FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.

Pathways

FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.

FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.
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Product protocols

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Target data

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
See full target information Fibroblast growth factor 21
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 22:e00501 PubMed39609161

2024

Intranasal administration of mesenchymal stem cells overexpressing FGF21 demonstrates therapeutic potential in experimental Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

You-Yen Lin,De-Maw Chuang,Cheng-Yu Chi,Shih-Ya Hung

Journal of cachexia, sarcopenia and muscle 15:2729-2742 PubMed39482887

2024

Sucla2 Knock-Out in Skeletal Muscle Yields Mouse Model of Mitochondrial Myopathy With Muscle Type-Specific Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Makayla S Lancaster,Paul Hafen,Andrew S Law,Catalina Matias,Timothy Meyer,Kathryn Fischer,Marcus Miller,Chunhai Hao,Patrick Gillespie,David McKinzie,Jeffrey J Brault,Brett H Graham

iScience 27:109839 PubMed39323737

2024

Kidney fibrosis molecular mechanisms Spp1 influences fibroblast activity through transforming growth factor beta smad signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Ding,Zidu Xu,Ying Lu,Qi Yuan,Jianzhong Li,Qi Sun

Molecular metabolism 86:101983 PubMed38960128

2024

Deletion of miPEP in adipocytes protects against obesity and insulin resistance by boosting muscle metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Alexis Diaz-Vegas,Kristen C Cooke,Harry B Cutler,Belinda Yau,Stewart W C Masson,Dylan Harney,Oliver K Fuller,Meg Potter,Søren Madsen,Niamh R Craw,Yiju Zhang,Cesar L Moreno,Melkam A Kebede,G Gregory Neely,Jacqueline Stöckli,James G Burchfield,David E James

Parasitology research 122:3213-3231 PubMed37874393

2023

Effects of metformin on parasitological, pathological changes in the brain and liver and immunological aspects during visceral toxocariasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Amina M Salama,Rasha A Elmahy,Hoda A Ibrahim,Alaa Ibrahim Mohamed Amer,Asmaa Fawzy Eltantawy,Dina I Elgendy

Inflammation 47:209-226 PubMed37864659

2023

Fibroblast Growth Factor 21 Relieves Lipopolysaccharide-Induced Acute Lung Injury by Suppressing JAK2/STAT3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengsi Cai,Huihui Ye,Xiayan Zhu,Xiuchun Li,Luqiong Cai,Jiajia Jin,Qiwen Chen,Yuzhe Shi,Lehe Yang,Liangxing Wang,Xiaoying Huang

Molecular metabolism 72:101718 PubMed37030441

2023

Fibroblast growth factor-21 is required for weight loss induced by the glucagon-like peptide-1 receptor agonist liraglutide in male mice fed high carbohydrate diets.

Applications

Unspecified application

Species

Unspecified reactive species

Thao D V Le,Payam Fathi,Amanda B Watters,Blair J Ellis,Gai-Linn K Besing,Nadejda Bozadjieva-Kramer,Misty B Perez,Andrew I Sullivan,Jesse P Rose,Laurie L Baggio,Jacqueline Koehler,Jennifer L Brown,Michelle B Bales,Kaitlyn G Nwaba,Jonathan E Campbell,Daniel J Drucker,Matthew J Potthoff,Randy J Seeley,Julio E Ayala

Frontiers in endocrinology 14:1059020 PubMed36909316

2023

Fibroblast growth factor 21 is expressed and secreted from skeletal muscle following electrical stimulation extracellular ATP activation of the PI3K/Akt/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Arias-Calderón,Mariana Casas,Julián Balanta-Melo,Camilo Morales-Jiménez,Nadia Hernández,Paola Llanos,Enrique Jaimovich,Sonja Buvinic

Molecular metabolism 63:101535 PubMed35760318

2022

Adipocyte-specific ablation of the Ca pump SERCA2 impairs whole-body metabolic function and reveals the diverse metabolic flexibility of white and brown adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Bauzá-Thorbrügge,Elin Banke,Belén Chanclón,Eduard Peris,Yanling Wu,Saliha Musovic,Cecilia Jönsson,Peter Strålfors,Patrik Rorsman,Charlotta S Olofsson,Ingrid Wernstedt Asterholm

Life science alliance 5: PubMed35459739

2022

Adipose tissue-specific ablation of Ces1d causes metabolic dysregulation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Li,Xin Li,Li Yang,Shuyue Wang,Yulin Dai,Baharan Fekry,Lucas Veillon,Lin Tan,Rebecca Berdeaux,Kristin Eckel-Mahan,Philip L Lorenzi,Zhongming Zhao,Richard Lehner,Kai Sun
View all publications
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