Mouse FGF-21 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse FGF-21 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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Publications
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- Cell reports. Medicine 3:1008102022Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid C D Hope,Charlotte E Hinds,Tatiana Lopes,Matthew L Vincent,Jed V Shrewsbury,Arthur T C Yu,Iona Davies,Rebecca Scott,Ben Jones,Kevin G Murphy,James S Minnion,Alessandro Sardini,David Carling,Thomas A Lutz,Stephen R Bloom,Tricia M M Tan,Bryn M OwenPubMed 36384093
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.2 - 4800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.
Alternative names
Fibroblast growth factor 21, FGF-21, Fgf21
What's included?
Recommended products
Mouse FGF-21 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse FGF-21 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 1.2 - 4800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 94 | Range 91 - 97 % |
Sample type Serum | Average % = 103 | Range 99 - 107 % |
Sample type EDTA Plasma | Average % = 100 | Range 92 - 108 % |
Sample type Heparin Plasma | Average % = 94 | Range 88 - 104 % |
Sample type Citrate plasma | Average % = 101 | Range 94 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
FGF-21 in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FGF-21 protein in mouse serum, plasma, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Rat serum and plasma samples are not compatible with this kit.
FGF-21 is a secreted protein, a member of a large FGF gene family. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression but not via SLC2A4/GLUT4 expression. FGF-21 activity probably requires the presence of KLB.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.
- Biological function summary
FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.
- Pathways
FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.
- Associated diseases and disorders
FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.
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4 product images
Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (ab229382)
Example of mouse FGF-21 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (ab229382)
Interpolated concentrations of native FGF-21 in mouse serum and plasma samples.
The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor (to neat sample) corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 409 pg/mL in neat serum, 397 pg/mL in neat plasma (citrate), 358 pg/mL in neat plasma (EDTA), and 348 pg/mL in neat plasma (heparin).
Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (ab229382)
Interpolated concentrations of native FGF-21 in untreated L-929 cell culture supernatant samples.
The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows: L-929 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 59 pg/mL in neat L-929 cell culture supernatant.
Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (ab229382)
Interpolated concentrations of FGF-21 in normal female CD-1 mouse serum and pregnant female CD-1 mouse serum at day 18 of gestation.
The concentrations of FGF-21 were measured in three different dilutions in duplicate and interpolated from the FGF-21 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in pg FGF-21 per mL of neat serum (mean +/- SD, n=3). FGF-21 concentration was determined to be 401 pg/mg in normal mouse serum, and 9,193 pg/mL in pregnant mouse serum.
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