AB229382

Mouse FGF-21 ELISA Kit, Fluorescent

CatchPoint SimpleStep ELISA
Recombinant
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(1 Publication)

Mouse FGF-21 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Mouse FGF-21 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

Fibroblast growth factor 21, FGF-21, Fgf21

4 Images

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Interpolated concentrations of FGF-21 in normal female CD-1 mouse serum and pregnant female CD-1 mouse serum at day 18 of gestation.

The concentrations of FGF-21 were measured in three different dilutions in duplicate and interpolated from the FGF-21 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in pg FGF-21 per mL of neat serum (mean +/- SD, n=3). FGF-21 concentration was determined to be 401 pg/mg in normal mouse serum, and 9,193 pg/mL in pregnant mouse serum.

sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Example of mouse FGF-21 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Interpolated concentrations of native FGF-21 in untreated L-929 cell culture supernatant samples.

The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : L-929 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 59 pg/mL in neat L-929 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Interpolated concentrations of native FGF-21 in mouse serum and plasma samples.

The concentrations of FGF-21 were measured in duplicates, interpolated from the FGF-21 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 50%. The interpolated dilution factor (to neat sample) corrected values are plotted (mean +/- SD, n=2). The mean FGF-21 concentration was determined to be 409 pg/mL in neat serum, 397 pg/mL in neat plasma (citrate), 358 pg/mL in neat plasma (EDTA), and 348 pg/mL in neat plasma (heparin).

Key facts

Detection method

Fluorescent

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1 pg/mL

Range

1.2 - 4800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

FGF-21 in vitro CatchPoint^® SimpleStep ELISA^® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FGF-21 protein in mouse serum, plasma, and cell culture supernatants.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Rat serum and plasma samples are not compatible with this kit.

FGF-21 is a secreted protein, a member of a large FGF gene family. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression but not via SLC2A4/GLUT4 expression. FGF-21 activity probably requires the presence of KLB.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 97 %", "average": "= 94" }, { "sample": "Serum", "range": "99 - 107 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "92 - 108 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "88 - 104 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "94 - 106 %", "average": "= 101" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab229382-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB229382-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR", "number":"AB229382-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse FGF-21 Capture Antibody", "number":"AB229382-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB229382-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse FGF-21 Lyophilized Recombinant Protein", "number":"AB229382-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB229382-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB229382-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB229382-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB229382-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse FGF-21 Detector Antibody", "number":"AB229382-CMP11", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB229382-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGF21 or fibroblast growth factor 21 is an important metabolic regulator. It is a member of the fibroblast growth factor family but unlike many others FGF21 functions mainly in endocrine signaling. The molecular weight of FGF21 is approximately 22 kDa. This protein is expressed in tissues such as the liver adipose tissue and pancreas. Because of its endocrine roles FGF21 travels through the bloodstream to exert its functions on distant organs.

Biological function summary

FGF21 influences glucose and lipid metabolism. It does not act as part of a larger protein complex. Instead it directly affects metabolic processes enhancing insulin sensitivity and lipid oxidation while reducing body weight. These actions help maintain energy balance and homeostasis making FGF21 an important player in metabolic control.

Pathways

FGF21 plays a significant role in the insulin signaling pathway and PPARγ coactivator 1α (PGC-1α) pathway. These pathways regulate cellular energy metabolism and mitochondrial biogenesis respectively. FGF21 interacts with other molecules such as PPARα (peroxisome proliferator-activated receptor alpha) to exert its effects demonstrating its integration into broader metabolic networks. FGF21's interaction with these pathways highlights its function in energy utilization and storage.

FGF21 has connections to conditions such as obesity and type 2 diabetes. Its role in improving insulin sensitivity and promoting lipid oxidation makes it a molecule of interest in combating metabolic syndrome. FGF21 also has a relationship with hepatic proteins such as insulin receptor substrates (IRS) which play roles in liver functioning. Understanding these relationships offers insights into potential therapeutic strategies targeting metabolic disorders.

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Product protocols

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Target data

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity probably requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity.

See full target information Fibroblast growth factor 21

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 3:100810 PubMed36384093

2022

Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss.

Applications

Unspecified application

Species

Unspecified reactive species

David C D Hope,Charlotte E Hinds,Tatiana Lopes,Matthew L Vincent,Jed V Shrewsbury,Arthur T C Yu,Iona Davies,Rebecca Scott,Ben Jones,Kevin G Murphy,James S Minnion,Alessandro Sardini,David Carling,Thomas A Lutz,Stephen R Bloom,Tricia M M Tan,Bryn M Owen

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse FGF-21 ELISA Kit, Fluorescent

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Sandwich ELISA - Mouse FGF-21 ELISA Kit, Fluorescent (AB229382)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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