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AB283884

Mouse FGL2/Prothrombinase ELISA Kit

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Mouse FGL2/Prothrombinase ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse FGL2/Prothrombinase with a sensitivity of 0.03 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Fiblp, Fgl2, Fibroleukin, Cytotoxic T-lymphocyte-specific protein, Fibrinogen-like protein 2, Prothrombinase

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Sandwich ELISA - Mouse FGL2/Prothrombinase ELISA Kit (AB283884)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGL2/Prothrombinase ELISA Kit (AB283884)

Interpolated concentrations of native FGL2/Prothrombinase in mouse serum, plasma (EDTA), plasma (citrate), and plasma (heparin).

The concentrations of FGL2/Prothrombinase were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (EDTA) 50%, plasma (citrate) 6.3%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 37.3 ng/mL in neat serum, 20.3 ng/mL in neat plasma (EDTA), 45.8 ng/mL in neat plasma (citrate), and 34.0 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse FGL2/Prothrombinase ELISA Kit (AB283884)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse FGL2/Prothrombinase ELISA Kit (AB283884)

Example of mouse FGL2/Prothrombinase standard curve in Sample Diluent NS.

The FGL2/Prothrombinase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.03 ng/mL

Range

0.188 - 12 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB198029

Anti-FGL2/Prothrombinase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL2/Prothrombinase antibody (AB198029)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Proteins & Peptides

AB161182

Recombinant Human FGL2/Prothrombinase protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human FGL2/Prothrombinase protein (GST tag N-Terminus) (AB161182)

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Primary Antibodies

AB275091

Anti-FGL1 antibody [EPR24018-27]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-FGL1 antibody [EPR24018-27] (AB275091)

  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

MouseFGL2/Prothrombinase ELISA Kit (ab283884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGL2/Prothrombinase protein in serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FGL2/Prothrombinase with 0.03 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

FGL2 (Fibrinogen-Like Protein 2, also known as Fibroleukin and Prothrombinase exists as both membrane bound and secreted by cytotoxic and helper T-cells (CD8+ and CD4+). By converting prothrombin to thrombin, it contributes to physiologic lymphocyte functions at mucosal sites.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 108 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "81 - 88 %", "average": "= 84" }, { "sample": "Cell culture media", "range": "87 - 106 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "88 - 90 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "90 - 109 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FGL2 also known as Fibroleukin or Prothrombinase is a protein involved in various physiological and pathological processes. Its molecular mass is approximately 70 kDa. This protein is widely expressed in different tissues including the liver and the spleen as well as in immune cells such as T cells and macrophages. It serves as an important signaling molecule and mediator in various biological pathways due to its capacity to interact with other proteins and molecular complexes.
Biological function summary

FGL2 acts in immune response modulation and blood coagulation. It can exist as a homodimer or form part of a larger protein complex. One of its roles involves cleaving prothrombin to generate thrombin contributing to the coagulation cascade. Moreover FGL2 has immunosuppressive properties affecting T cell proliferation and cytokine release. Its dual function in coagulation and immune regulation points to its importance in maintaining homeostasis and orchestrating immune responses.

Pathways

FGL2 serves key roles in both the coagulation and immune pathways. In the coagulation pathway FGL2 aids in the conversion of prothrombin to thrombin bridging innate immunity with coagulation processes. This action places it in close functional relationship with proteins in the coagulation pathway such as thrombin and fibrin. Moreover its involvement in immune pathways aligns it with cytokines and interferons modulating the immune response and maintaining immune system balance.

FGL2 plays significant roles in conditions such as viral hepatitis and certain autoimmune disorders. In viral hepatitis FGL2's upregulation contributes to liver fibrosis and inflammation often in conjunction with another protein CD147. In autoimmune disorders the dysregulation of FGL2 can lead to excessive inflammatory responses impacting proteins like the interferon-gamma. Understanding FGL2's involvement in these diseases can inform therapeutic strategies targeting both coagulation and immune dysregulation.
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Product protocols

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Target data

Converts prothrombin to thrombin.
See full target information Fgl2
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websiteProtocolBooklet
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Product promise

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