AB108843
Mouse Fibrinogen ELISA Kit is a sandwich ELISA designed to quantify Mouse Fibrinogen with a sensitivity of 0.12 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.5 - 12.5 ng/mL
View Alternative Names
Fibrinogen gamma chain, Fgg
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Mouse Fibrinogen ELISA Kit (AB108843)
Representative Standard Curve using ab108843
Reactivity data
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Product details
Mouse Fibrinogen ELISA Kit ab108843 is a sandwich ELISA to measure Mouse Fibrinogen in plasma, urine with a sensitivity of 0.12 ng/ml.
How the assay works
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Fibrinogen ELISA Kit ab108843 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
Get results in 90 minutes with our SimpleStep ELISA® kit
Mouse Fibrinogen ELISA Kit (ab213478)
How the assay works
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibrinogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Fibrinogen ELISA Kit ab108843 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
Get results in 90 minutes with our SimpleStep ELISA® kit
Mouse Fibrinogen ELISA Kit (ab213478)
Precision
[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix (By similarity). Fibrin has a major function in hemostasis as one of the primary components of blood clots (By similarity). In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization (By similarity). Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo (By similarity). Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway (PubMed : 19332769). Maternal fibrinogen is essential for successful pregnancy (By similarity). Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage (By similarity). May also facilitate the immune response via both innate and T-cell mediated pathways (By similarity).
Additional targets
Fgb,Fibrinogen gamma chain
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
PloS one 9:e110980 PubMed25333937
2014
New candidate biomarkers in the female genital tract to evaluate microbicide toxicity.
Applications
Unspecified application
Species
Unspecified reactive species
Scott Fields,Benben Song,Bareza Rasoul,Julie Fong,Melissa G Works,Kenneth Shew,Ying Yiu,Jon Mirsalis,Annalisa D'Andrea
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