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AB108844

Mouse Fibrinogen ELISA Kit

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(4 Publications)

Mouse Fibrinogen ELISA Kit is a Competitive ELISA for the measurement of Mouse Fibrinogen in Mouse in Biofluids samples.

View Alternative Names

Fibrinogen beta chain, Fgb

2 Images
Competitive ELISA - Mouse Fibrinogen ELISA Kit (AB108844)
  • cELISA

Unknown

Competitive ELISA - Mouse Fibrinogen ELISA Kit (AB108844)

Fibrinogen measured in biological fluids showing quantity (ug) per mL of tested sample. Samples diluted 1-500 fold.

Competitive ELISA - Mouse Fibrinogen ELISA Kit (AB108844)
  • cELISA

Supplier Data

Competitive ELISA - Mouse Fibrinogen ELISA Kit (AB108844)

Typical standard curve using ab108844

Key facts

Detection method

Colorimetric

Sample types

Plasma

Reacts with

Mouse

Assay type

Competitive

Sensitivity

= 1.1 µg/mL

Range

1.25 - 20 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Fibrinogen Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse fibrinogen in plasma.

A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108844-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108844-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Mouse Fibrinogen (Lyophilized)", "number":"AB108844-CMP10", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108844-CMP09", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108844-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108844-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibrinogen Microplate (12 x 8 well strips)", "number":"AB108844-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fibrinogen Standard", "number":"AB108844-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108844-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108844-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
See full target information Fgb
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 8:e11740 PubMed36439760

2022

B lymphocyte-deficiency in mice promotes venous thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Solveig Hasselwander,Ning Xia,Maximilian Mimmler,Stefanie Ascher,Tanja Knopp,Gisela Reifenberg,Susanne Karbach,Wolfram Ruf,Christoph Reinhardt,Huige Li

Cell death & disease 13:722 PubMed35982051

2022

Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Yuan,Kelong Ai,Menghua Xiang,Chengliang Xie,Mingyi Zhao,Ming Wu,Hongli Li,Yueren Wu,Yueqing Cao,Can Li,Yanjun Zhong,Xiaomeng Pei,Helen Ka Wai Law,Liqian Gao,Qicai Xiao,Xinyu Yang

TH open : companion journal to thrombosis and haem 4:e66-e76 PubMed32309772

2020

Assessment of Thrombotic and Bleeding Tendency in Two Mouse Models of Chronic Kidney Disease: Adenine-Diet and 5/6th Nephrectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Camélia Makhloufi,Lydie Crescence,Roxane Darbousset,Nathalie McKay,Ziad A Massy,Christophe Dubois,Laurence Panicot-Dubois,Stéphane Burtey,Stéphane Poitevin

Stroke 45:1453-9 PubMed24676774

2014

Mannose-binding lectin promotes local microvascular thrombosis after transient brain ischemia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier de la Rosa,Alvaro Cervera,Anna K Kristoffersen,Claudia P Valdés,Hari M Varma,Carles Justicia,Turgut Durduran,Ángel Chamorro,Anna M Planas
View all publications
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websiteProtocolBooklet
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Product promise

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