Mouse Fibrinogen ELISA Kit is a Competitive ELISA kit for the measurement of Mouse Fibrinogen in Mouse in Plasma samples.
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- Heliyon 8:e117402022B lymphocyte-deficiency in mice promotes venous thrombosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSolveig Hasselwander,Ning Xia,Maximilian Mimmler,Stefanie Ascher,Tanja Knopp,Gisela Reifenberg,Susanne Karbach,Wolfram Ruf,Christoph Reinhardt,Huige LiPubMed 36439760
- Cell death & disease 13:7222022Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuang Yuan,Kelong Ai,Menghua Xiang,Chengliang Xie,Mingyi Zhao,Ming Wu,Hongli Li,Yueren Wu,Yueqing Cao,Can Li,Yanjun Zhong,Xiaomeng Pei,Helen Ka Wai Law,Liqian Gao,Qicai Xiao,Xinyu YangPubMed 35982051
- TH open : companion journal to thrombosis and haem 4:e66-e762020Assessment of Thrombotic and Bleeding Tendency in Two Mouse Models of Chronic Kidney Disease: Adenine-Diet and 5/6th Nephrectomy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamélia Makhloufi et. al.PubMed 32309772
- Stroke 45:1453-92014Mannose-binding lectin promotes local microvascular thrombosis after transient brain ischemia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXavier de la Rosa et. al.PubMed 24676774
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma
- Assay type
- Competitive
- Reactive species
- Mouse
- Range
- 0.625 - 20 µg/mL
- Assay time
- 3h
- Sensitivity
- = 1.1 µg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.
Alternative names
Fibrinogen beta chain, Fgb
What's included?
Recommended products
Mouse Fibrinogen ELISA Kit is a Competitive ELISA kit for the measurement of Mouse Fibrinogen in Mouse in Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma
- Assay type
- Competitive
- Reactive species
- Mouse
- Range
- 0.625 - 20 µg/mL
- Assay time
- 3h
- Assay Platform
- Microplate
- Sensitivity
- = 1.1 µg/mL
- General recovery
- 93%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 5.2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Fibrinogen Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse fibrinogen in plasma.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Competitive ELISA - Mouse Fibrinogen ELISA Kit (ab108844)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Competitive ELISA - Mouse Fibrinogen ELISA Kit (ab108844)
Fibrinogen measured in biological fluids showing quantity (ug) per mL of tested sample. Samples diluted 1-500 fold.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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