Mouse Fibronectin ELISA Kit is a sandwich ELISA designed to quantify Mouse Fibronectin with a sensitivity of 0.91 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 100 ng/mL
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Publications
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- Journal of cellular and molecular medicine 27:2228-22382023Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillaume Postic,Jean Solarz,Cécile Loubière,Janany Kandiah,Jaysen Sawmynaden,Frederic Adam,Marie Vilaire,Thibaut Léger,Jean-Michel Camadro,Daniella Balduino Victorino,Marie-Claude Potier,Eric Bun,Gautier Moroy,Alexandre Kauskot,Olivier Christophe,Nathalie JanelPubMed 37415307
- Nature methods 19:1449-14602022Geometric engineering of organoid culture for enhanced organogenesis in a dish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun HuhPubMed 36280722
- Journal of cellular physiology 236:249-2592020Fibroblast growth factor inducible 14 signaling facilitates anti-dsDNA IgG penetration into mesangial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuilian Li,Fangyan Jia,Kaixuan Ren,Mai Luo,Xiaoyun Min,Shengxiang Xiao,Yumin XiaPubMed 32542768
- Molecular & cellular proteomics : MCP 15:1681-912016Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Hyun Jo,Jingi Bae,Sehyun Chae,Jin Hyoung Kim,Jong-Hee Han,Daehee Hwang,Sang-Won Lee,Jeong Hun KimPubMed 26969716
- Cancer research 76:347-572016Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuta Kasagi et. al.PubMed 26744523
- Journal of cell science 127:2217-262014Procaspase-3 regulates fibronectin secretion and influences adhesion, migration and survival independently of catalytic function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Brentnall et. al.PubMed 24610949
- Laboratory investigation; a journal of technical m 93:31-402012β1 integrin-extracellular matrix interactions are essential for maintaining exocrine pancreas architecture and function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew M Riopel et. al.PubMed 23069938
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 6.25 - 100 ng/mL
- Assay time
- 3h 30m
- Sensitivity
- = 0.91 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (By similarity). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (PubMed:21768292). Participates in the regulation of type I collagen deposition by osteoblasts (PubMed:21768292). Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation (PubMed:34089617). Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Alternative names
Fibronectin, FN, Fn1
What's included?
Recommended products
Mouse Fibronectin ELISA Kit is a sandwich ELISA designed to quantify Mouse Fibronectin with a sensitivity of 0.91 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 100 ng/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 6.25 - 100 ng/mL
- Assay time
- 3h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 0.91 ng/mL
- General recovery
- 96%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Mouse Fibronectin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108849) is designed for the quantitative measurement of Fibronectin levels in plasma, serum and urine samples.
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Sandwich ELISA - Mouse Fibronectin ELISA Kit (ab108849)
Fibronectin measured in biological fluids showing quantity (ng) per mL of sample tested
Sandwich ELISA - Mouse Fibronectin ELISA Kit (ab108849)
Representative Standard Curve using ab108849
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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