AB108849
Mouse Fibronectin ELISA Kit is a sandwich ELISA designed to quantify Mouse Fibronectin with a sensitivity of 0.91 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 100 ng/mL
View Alternative Names
Fibronectin, FN, Fn1
2 Images
- sELISA
Lab
Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB108849)
Fibronectin measured in biological fluids showing quantity (ng) per mL of sample tested
- sELISA
Supplier Data
Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB108849)
Representative Standard Curve using ab108849
Complementary Products
Reactivity data
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Product details
Mouse Fibronectin ELISA Kit ab108849 is a sandwich ELISA to measure Mouse Fibronectin in serum, plasma, urine with a sensitivity of 0.91 ng/ml.
How the assay works
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Fibronectin ELISA Kit ab108849 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
How the assay works
A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Fibronectin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Fibronectin captured in plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Fibronectin ELISA Kit ab108849 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
Target data
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (By similarity). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (PubMed : 21768292). Participates in the regulation of type I collagen deposition by osteoblasts (PubMed : 21768292). Acts as a ligand for the Lilrb4a receptor, inhibiting Fcgr1/CD64-mediated monocyte activation (PubMed : 34089617).. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Publications (8)
Recent publications for all applications. Explore the full list and refine your search
Physiological reports 13:e70253 PubMed39980189
2025
Lung-delivered IL-10 mitigates Lung inflammation induced by repeated endotoxin exposures in male mice.
Applications
Unspecified application
Species
Unspecified reactive species
Aaron D Schwab,Todd A Wyatt,Oliver W Schanze,Amy J Nelson,Angela M Gleason,Michael J Duryee,Deanna D Mosley,Geoffrey M Thiele,Ted R Mikuls,Jill A Poole
Journal of cellular and molecular medicine 27:2228-2238 PubMed37415307
2023
Over-expression of Dyrk1A affects bleeding by modulating plasma fibronectin and fibrinogen level in mice.
Applications
Unspecified application
Species
Unspecified reactive species
Guillaume Postic,Jean Solarz,Cécile Loubière,Janany Kandiah,Jaysen Sawmynaden,Frederic Adam,Marie Vilaire,Thibaut Léger,Jean-Michel Camadro,Daniella Balduino Victorino,Marie-Claude Potier,Eric Bun,Gautier Moroy,Alexandre Kauskot,Olivier Christophe,Nathalie Janel
Nature methods 19:1449-1460 PubMed36280722
2022
Geometric engineering of organoid culture for enhanced organogenesis in a dish.
Applications
Unspecified application
Species
Unspecified reactive species
Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh
Journal of cellular physiology 236:249-259 PubMed32542768
2020
Fibroblast growth factor inducible 14 signaling facilitates anti-dsDNA IgG penetration into mesangial cells.
Applications
Unspecified application
Species
Unspecified reactive species
Ruilian Li,Fangyan Jia,Kaixuan Ren,Mai Luo,Xiaoyun Min,Shengxiang Xiao,Yumin Xia
Molecular & cellular proteomics : MCP 15:1681-91 PubMed26969716
2016
Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability.
Applications
Unspecified application
Species
Unspecified reactive species
Dong Hyun Jo,Jingi Bae,Sehyun Chae,Jin Hyoung Kim,Jong-Hee Han,Daehee Hwang,Sang-Won Lee,Jeong Hun Kim
Cancer research 76:347-57 PubMed26744523
2016
Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix-Directed Spheroid Formation.
Applications
Unspecified application
Species
Unspecified reactive species
Yuta Kasagi,Yui Harada,Yosuke Morodomi,Toshiki Iwai,Satoru Saito,Kumi Yoshida,Eiji Oki,Hiroshi Saeki,Kippei Ohgaki,Masahiko Sugiyama,Mitsuho Onimaru,Yoshihiko Maehara,Yoshikazu Yonemitsu
Journal of cell science 127:2217-26 PubMed24610949
2014
Procaspase-3 regulates fibronectin secretion and influences adhesion, migration and survival independently of catalytic function.
Applications
Unspecified application
Species
Unspecified reactive species
Matthew Brentnall,David B Weir,Anthony Rongvaux,Adam I Marcus,Lawrence H Boise
Laboratory investigation; a journal of technical m 93:31-40 PubMed23069938
2012
β1 integrin-extracellular matrix interactions are essential for maintaining exocrine pancreas architecture and function.
Applications
Unspecified application
Species
Unspecified reactive species
Matthew M Riopel,Jinming Li,Shangxi Liu,Andrew Leask,Rennian Wang
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