Mouse Fibronectin ELISA Kit

• ELISA

Supplier Data

ELISA - Mouse Fibronectin ELISA Kit (AB210967)

• sELISA

Lab

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Example of mouse Fibronectin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Lab

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Interpolated concentration of native Fibronectin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1/4000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

• sELISA

Supplier Data

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Example of mouse Fibronectin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Comparison of secreted Fibronectin in unstimulated and PMA/PHA-stimulated J774A.1 Cells.

J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Fibronectin was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated J774A.1 and cell culture media. Measured values were interpolated from the Fibronectin Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 294 ng/mL unstimulated, 27.3 ng/mL stimulated, and undetectable in media.

• sELISA

Supplier Data

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Interpolated concentrations of native Fibronectin in mouse urine and cell culture supernatant samples.

The concentrations of Fibronectin were measured in duplicates, interpolated from the Fibronectin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 5% and L929 supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 979 ng/mL in urine and 300 ng/mL in L929 supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Comparison of secreted Fibronectin in unstimulated and PMA/PHA-stimulated L929 Cells.

L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Fibronectin was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Fibronectin Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 300 ng/mL unstimulated, 2,209 ng/mL stimulated, and undetectable in media.

• sELISA

Supplier Data

Sandwich ELISA - Mouse Fibronectin ELISA Kit (AB210967)

Interpolated concentrations of native Fibronectin in mouse serum and plasma samples.

The concentrations of Fibronectin were measured in duplicates, interpolated from the Fibronectin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (citrate) 1 : 4,000, plasma (EDTA) 1 : 8,000, and plasma (heparin) 1 : 10,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibronectin concentration was determined to be 100 μg/mL in serum, 172 μg/mL in plasma (citrate), 220 μg/mL in plasma (EDTA) and 211 μg/mL in plasma (heparin).