Mouse Flt-3 ELISA Kit
- Recombinant
- SimpleStep
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Mouse Flt-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Flt-3 with a sensitivity of 0.915 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD135, Flk-2, Flt-3, Flt3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase 2, Fms-like tyrosine kinase 3, Tyrosine-protein kinase receptor flk-2, FLK-2, FLT-3
- sELISA
Supplier Data
Sandwich ELISA - Mouse Flt-3 ELISA Kit (AB256395)
Example of mouse Flt-3 standard curve in 1X Cell Extraction Buffer PTR.
The Flt-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Flt-3 ELISA Kit (AB256395)
Interpolated concentrations of native Flt-3 in mouse M1 cell extract based on a 200 μg/mL extract load.
The concentrations of Flt-3 were measured in duplicate and interpolated from the Flt-3 standard curve and corrected for sample dilution. The interpolated, dilution factor corrected, values are plotted (mean +/- SD, n=2). The mean Flt-3 concentration was determined to be 40.5 ng/mL.
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Product details
Mouse Flt-3 ELISA Kit (ab256395) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Flt-3 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Flt-3 with 0.915 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Flt-3, also known as CD135, is a receptor type tyrosine kinase involved in the differentiation of hematopoietic cells. Binding of its ligand, FLT3L induces phosphorylation cascades through multiple kinases, including STAT5A and MAPK1. Flt-3 is used to differentiate multipotent progenitor cells and hematopoietic stem cells. Mutations in this gene are linked to acute myelogenous leukemia and its levels are negatively associated with survival. Given Flt-3’s importance in this disease, it is currently being investigated as a therapeutic target.
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Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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