Mouse Flt3 ligand ELISA Kit (Flt3L)

Product details

Mouse FLT3 Ligand ELISA kit (ab275551) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse FLT3 Ligand protein in serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FLT3 Ligand with 3.9 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Flt-3 ligand is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 ligand consists of a 158 amino acid extracellular domain with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Flt-3 ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble Flt-3 ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. Animal studies report Flt-3 ligand to reduce the severity of experimentally induced allergic inflammation. Human Flt-3 ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively.