AB283540

Mouse FRZB ELISA Kit

Recombinant
SimpleStep
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Mouse FRZB ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse FRZB with a sensitivity of 96.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Fiz, Fre, Frzb1, Sfrp3, Frzb, Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1

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Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

sELISA

Supplier Data

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

Interpolated concentrations of native FRZB in mouse serum samples.

The concentrations of FRZB were measured in duplicates, interpolated from the FRZB standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FRZB concentration was determined to be 1,085.38 pg/mL in mouse serum.

sELISA

Supplier Data

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

Example of mouse FRZB standard curve in Sample Diluent NS.

The FRZB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

Interpolated concentrations of spiked FRZB in mouse serum, plasma, and cell culture media samples.

The concentrations of FRZB were measured in duplicates, interpolated from the FRZB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and 1640 RPMI cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 96.99 pg/mL

Range

140.63 - 9000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse FRZB ELISA kit (ab283540) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse FRZB protein in mouse serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FRZB with 96.99 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

FRZB is a Wnt-binding regulatory protein involved in embryonic development, the regulation of chondrocyte maturation, and long bone development. Osteoarthritis and Holzgreve Syndrome are associated with mutations the FRZB protein. FRZB knockout mice have increased cortical thickness.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.6" } ]

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Recovery

[ { "sample": "Serum", "range": "91 - 99 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "102 - 123 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "94 - 100 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "107 - 117 %", "average": "= 114" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283540-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283540-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283540-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283540-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB283540-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283540-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse FRZB Detector Antibody", "number":"AB283540-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283540-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283540-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse FRZB Capture Antibody", "number":"AB283540-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse FRZB Lyophilized Recombinant Protein", "number":"AB283540-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FRZB also known as secreted frizzled-related protein 3 (SFRP3) functions as a modulator of Wnt signaling pathways. This protein has a mass of approximately 36 kDa and serves as an antagonist to the Wnt proteins by binding to them preventing their interaction with cell surface receptors. FRZB is expressed in several tissues with notable levels in cartilage bone and the heart.

Biological function summary

The modulation of Wnt signaling by FRZB plays a significant role in embryonic development and tissue homeostasis. FRZB is not part of a protein complex but has important interactions with other components of the signaling cascade. It influences cell differentiation proliferation and apoptosis which are critical processes during development and tissue repair.

Pathways

FRZB influences both Wnt/β-catenin and non-canonical Wnt pathways. Its interaction with these pathways regulates signal transmission that affects cellular behaviors. Related proteins include Wnt ligands and frizzled receptors which are central to these signaling mechanisms.

Abnormalities in FRZB expression or function show a connection to osteoarthritis and certain cancers. In osteoarthritis FRZB interacts with Wnt proteins which contribute to the degradation of cartilage. In cancer dysregulation of Wnt signaling involving FRZB can lead to unchecked cell proliferation. Understanding its role in these diseases can aid in developing therapeutic strategies.

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Product protocols

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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development (By similarity).

See full target information Frzb

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse FRZB ELISA Kit

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

Sandwich ELISA - Mouse FRZB ELISA Kit (AB283540)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Sample Prep & Detection Kits

Primary Antibodies

Assay Kits

Product promise