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AB277437

Mouse FSTL1/FRP ELISA Kit

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Mouse FSTL1/FRP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse FSTL1/FRP in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Frp, Fstl, Tsc36, Fstl1, Follistatin-related protein 1, Follistatin-like protein 1, TGF-beta-inducible protein TSC-36

1 Images
ELISA - Mouse FSTL1/FRP ELISA Kit (AB277437)
  • ELISA

Supplier Data

ELISA - Mouse FSTL1/FRP ELISA Kit (AB277437)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.43 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse FSTL1/FRP ELISA Kit (ab277437) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FSTL1/FRP in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse FSTL1/FRP coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse FSTL1/FRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse FSTL1/FRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse FSTL1/FRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "72 - 85 %", "average": "= 76.47" }, { "sample": "Plasma", "range": "70 - 104 %", "average": "= 78.87" }, { "sample": "Cell culture media", "range": "70 - 97 %", "average": "= 81.04" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277437-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277437-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277437-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse FSTL1 FRP coated Microplate", "number":"AB277437-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse FSTL1/FRP standard protein (Lyophilized)", "number":"AB277437-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277437-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse FSTL1/FRP Antibody", "number":"AB277437-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277437-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277437-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FSTL1 also known as FRP is a protein that plays an important role in various cellular functions. The protein is a glycoprotein with a molecular mass of approximately 32 kDa and is expressed in a variety of tissues including heart lung and placenta. FSTL1 contains several domains that facilitate its interaction with other proteins and cellular components. It is located both intracellularly and secreted in the extracellular matrix contributing to its role in cell signaling and structural integrity.
Biological function summary

FSTL1 serves as a modulator in inflammatory and developmental processes. It is a part of a complex that regulates immune responses and cell growth. The protein has been shown to affect the activity of multiple growth factors including Bone Morphogenetic Proteins (BMPs) revealing its regulatory capacity in tissue development and repair. FSTL1 can influence cell differentiation proliferation and survival highlighting its significance in maintaining cellular homeostasis.

Pathways

FSTL1 has important roles in the regulation of the BMP signaling and Wnt pathways. In the BMP pathway FSTL1 interacts with other signaling molecules to modulate pathway intensity and cellular outcomes. The protein links with BMP proteins to influence processes like bone formation and tissue repair. In the Wnt pathway FSTL1 can modulate key interactions that affect cellular proliferation and development. Through these pathways FSTL1 also relates to proteins such as TGF-beta which participate in various cellular processes.

FSTL1 has relevance in conditions such as rheumatoid arthritis and pulmonary hypertension. The protein shows increased expression in inflammatory environments indicating its involvement in rheumatoid arthritis where it engages with immune response pathways. The connection with pulmonary hypertension also positions FSTL1 as a significant factor in cardiovascular pathologies. In both conditions FSTL1 interacts with cytokines and other immune-related proteins influencing disease progression and response to treatment.
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Product protocols

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Target data

Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (By similarity). Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter (PubMed : 19595790, PubMed : 21826198). Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes (PubMed : 20054002). Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors (By similarity).
See full target information Fstl1
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websiteProtocolBooklet
en
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Product promise

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